Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Disks large-associated protein 3 (DLGAP3) Standard Curve.)
Rat Disks Large-Associated Protein 3 (DLGAP3) ELISA Kit | DLGAP3 elisa kit
Rat Disks Large-Associated Protein 3 (DLGAP3) ELISA Kit
Gene Names
Dlgap3; Dap3; DAP-3; SAPAP3
Reactivity
Rat
Synonyms
Disks Large-Associated Protein 3 (DLGAP3); Rat Disks Large-Associated Protein 3 (DLGAP3) ELISA Kit; DLGAP3; DAP3; SAPAP3; OTTHUMP00000004180; PSD-95/SAP90-binding protein 3; SAP90/PSD 95 associated protein 3; SAP90/PSD-95-associated protein 3; disks large-associated protein 3; DLGAP3 elisa kit
Reactivity
Rat
Specificity
Rat Disks large-associated protein 3 (DLGAP3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLGAP3. No significant cross-reactivity or interference between Rat DLGAP3 and analogues was observed.
Sequence Length
977
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Disks large-associated protein 3 (DLGAP3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Disks large-associated protein 3 (DLGAP3) Standard Curve.)
Related Product Information for DLGAP3 elisa kit
Description: This Rat Disks large-associated protein 3 (DLGAP3) ELISA Kit employs a two-site sandwich ELISA to quantitate DLGAP3 in samples. An antibody specific for DLGAP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLGAP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLGAP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Using a segment of rat Fak (PTK2) to screen an adult human brain cDNA library, Bongiorno-Borbone et al. (2005) cloned DLGAP3, which they called SAPAP3. The deduced protein contains 977 amino acids. By Northern blot analysis, Takeuchi et al. (1997) found brain-specific expression of rat Sapap3. The rat protein localized to dendrites and cell bodies and was enriched in synaptosomal membrane fractions.By mutation analysis, Bongiorno-Borbone et al. (2005) demonstrated that the N-terminal half of human SAPAP3 interacted with a C-terminal segment of rat Fak. They also found that SAPAP3 interacted with Pyk2 (PTK2B) in in vitro pull-down assays. In rat forebrain lysates, part of Fak and Pyk2 had a similar subcellular distribution as Sapap3 and Sap90/Psd95, which supported their functional association.
Background: Using a segment of rat Fak (PTK2) to screen an adult human brain cDNA library, Bongiorno-Borbone et al. (2005) cloned DLGAP3, which they called SAPAP3. The deduced protein contains 977 amino acids. By Northern blot analysis, Takeuchi et al. (1997) found brain-specific expression of rat Sapap3. The rat protein localized to dendrites and cell bodies and was enriched in synaptosomal membrane fractions.By mutation analysis, Bongiorno-Borbone et al. (2005) demonstrated that the N-terminal half of human SAPAP3 interacted with a C-terminal segment of rat Fak. They also found that SAPAP3 interacted with Pyk2 (PTK2B) in in vitro pull-down assays. In rat forebrain lysates, part of Fak and Pyk2 had a similar subcellular distribution as Sapap3 and Sap90/Psd95, which supported their functional association.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Disks large-associated protein 3
NCBI Official Synonym Full Names
DLG associated protein 3
NCBI Official Symbol
Dlgap3
NCBI Official Synonym Symbols
Dap3; DAP-3; SAPAP3
NCBI Protein Information
disks large-associated protein 3
UniProt Protein Name
Disks large-associated protein 3
Protein Family
UniProt Gene Name
Dlgap3
UniProt Synonym Gene Names
Dap3; DAP-3; SAPAP3
UniProt Entry Name
DLGP3_RAT
NCBI Description
binds neuronal cell guanylate kinase PSD-95/SAP90; may play a role in post synaptic density structure and synaptic function [RGD, Feb 2006]
Research Articles on DLGAP3
Similar Products
Product Notes
The Rat DLGAP3 dlgap3 (Catalog #AAA9712420) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712420 ELISA Kit recognizes Rat DLGAP3. It is sometimes possible for the material contained within the vial of "Disks Large-Associated Protein 3 (DLGAP3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
