Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Disks large-associated protein 1 (DLGAP1) Standard Curve.)
Rat Disks Large-Associated Protein 1 (DLGAP1) ELISA Kit | DLGAP1 elisa kit
Rat Disks Large-Associated Protein 1 (DLGAP1) ELISA Kit
Reactivity
Rat
Synonyms
Disks Large-Associated Protein 1 (DLGAP1); Rat Disks Large-Associated Protein 1 (DLGAP1) ELISA Kit; DLGAP1; DAP-1; DAP-1-ALPHA; DAP-1-BETA; GKAP; MGC88156; SAPAP1; hGKAP; PSD-95/SAP90 binding protein 1; SAP90/PSD-95-associated protein 1; discs large homolog-associated protein 1; disks large-associated protei; DLGAP1 elisa kit
Reactivity
Rat
Specificity
Rat Disks large-associated protein 1 (DLGAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLGAP1. No significant cross-reactivity or interference between Rat DLGAP1 and analogues was observed.
Sequence Length
992
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Disks large-associated protein 1 (DLGAP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Disks large-associated protein 1 (DLGAP1) Standard Curve.)
Related Product Information for DLGAP1 elisa kit
Description: This Rat Disks large-associated protein 1 (DLGAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLGAP1 in samples. An antibody specific for DLGAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLGAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLGAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DLGAP1encodes the protein called guanylate kinase-associated protein (GKAP). GKAP binds to the SHANK and PSD-95 proteins fascilitating the assembly of the post synaptic density of neurons. Sequence analysis predicted that the 627-amino acid hydrophilic protein, which is 98% identical to the rat sequence, contains five 14-amino acid repeats in its N terminus. Binding analysis determined that the N-terminal region of DLGAP1 interacts with intact GK domains of all members of the DLG family, which share approximately 75% homology in the GK domain; DLGAP1 was found to interact less well with more distantly related MAGUKs. Immunoblot analysis showed expression of 95- and 130-kD DLGAP1 proteins in membranes from different regions of rat brain.
Background: DLGAP1encodes the protein called guanylate kinase-associated protein (GKAP). GKAP binds to the SHANK and PSD-95 proteins fascilitating the assembly of the post synaptic density of neurons. Sequence analysis predicted that the 627-amino acid hydrophilic protein, which is 98% identical to the rat sequence, contains five 14-amino acid repeats in its N terminus. Binding analysis determined that the N-terminal region of DLGAP1 interacts with intact GK domains of all members of the DLG family, which share approximately 75% homology in the GK domain; DLGAP1 was found to interact less well with more distantly related MAGUKs. Immunoblot analysis showed expression of 95- and 130-kD DLGAP1 proteins in membranes from different regions of rat brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
disks large-associated protein 1
UniProt Protein Name
Disks large-associated protein 1
Protein Family
UniProt Gene Name
Dlgap1
UniProt Synonym Gene Names
Gkap; DAP-1; rGKAP; SAPAP1
UniProt Entry Name
DLGP1_RAT
Uniprot Description
SAPAP1: Part of the postsynaptic scaffold in neuronal cells. Belongs to the SAPAP family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Cellular Component: postsynaptic membrane; postsynaptic density; synapse; cell junction
Molecular Function: protein domain specific binding; protein binding; protein complex binding
Biological Process: cell-cell signaling
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Product Notes
The Rat DLGAP1 dlgap1 (Catalog #AAA9712422) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712422 ELISA Kit recognizes Rat DLGAP1. It is sometimes possible for the material contained within the vial of "Disks Large-Associated Protein 1 (DLGAP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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