Typical Testing Data/Standard Curve (for reference only)
Mouse Cyclin-dependent kinase-like 5 (CDKL5) ELISA Kit | CDKL5 elisa kit
Mouse Cyclin-dependent kinase-like 5 (CDKL5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CDKL5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDKL5 antibody and an CDKL5-HRP conjugate. The assay sample and buffer are incubated together with CDKL5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDKL5 concentration since CDKL5 from samples and CDKL5-HRP conjugate compete for the anti-CDKL5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDKL5 from the sample, fewer sites are left to bind CDKL5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDKL5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of Ser/Thr protein kinase family and encodes a phosphorylated protein with protein kinase activity. Mutations in this gene have been associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
CDKL5: a protein kinase of the CDKL family. Mutations have been associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). Alternate transcriptional splice variants have been characterized.
Protein type: Kinase, protein; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Protein kinase, CMGC; CMGC group; CDKL family
Chromosomal Location of Human Ortholog: Xp22
Cellular Component: nucleoplasm; dendrite cytoplasm; cytoplasm; nucleus
Molecular Function: protein serine/threonine kinase activity; cyclin-dependent protein kinase activity; Rac GTPase binding; kinase activity; ATP binding; protein kinase activity
Biological Process: regulation of cell cycle; protein amino acid autophosphorylation; positive regulation of axon extension; neuron migration; regulation of dendrite development; positive regulation of dendrite morphogenesis; protein amino acid phosphorylation
Disease: Angelman Syndrome; Epileptic Encephalopathy, Early Infantile, 2
Research Articles on CDKL5
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Product Notes
The Mouse CDKL5 cdkl5 (Catalog #AAA7250132) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7250132 ELISA Kit recognizes Mouse CDKL5. It is sometimes possible for the material contained within the vial of "Cyclin-dependent kinase-like 5 (CDKL5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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