Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dopamine D2 receptor (D2R) Standard Curve.)
Human Dopamine D2 Receptor (D2R) ELISA Kit | D2R elisa kit
Human Dopamine D2 Receptor (D2R) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dopamine D2 receptor (D2R) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dopamine D2 receptor (D2R) Standard Curve.)
Background: D2 (DRD2). There is a short version of D2 (D2Sh) and a long version of D2 (D2Lh): The D2Sh are pre-synaptic situated, having modulatory functions (called autoreceptor, they regulate the neurotransmission by feed-back mechanisms, i.e., synthesis, storage and release of dopamine into the synaptic cleft). The D2Lh may have the classic function of a post-synaptic receptor, i.e., keep going on the neurotransmission (excitatory or inhibitory) once blocked by a receptor antagonist or stimulated by the endogenous neurotransmitter itself or a synthetic full or partial agonist.D2-like activation is coupled to the G protein Galphai, which subsequently increases phosphodiesterase activity. Phosphodiesterases break down cAMP, producing an inhibitory effect in neurons.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]
Uniprot Description
DRD2: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11); also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1
Chromosomal Location of Human Ortholog: 11q23
Cellular Component: synaptic vesicle membrane; axon; endocytic vesicle; integral to plasma membrane; postsynaptic density; dendrite; plasma membrane; acrosome; dendritic spine; perikaryon; nerve terminal; lateral plasma membrane
Molecular Function: dopamine D2 receptor-like receptor activity; ionotropic glutamate receptor binding; identical protein binding; protein binding; potassium channel regulator activity; protein homodimerization activity; protein heterodimerization activity; dopamine binding; drug binding
Biological Process: response to nicotine; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of dopamine uptake; prepulse inhibition; response to toxin; positive regulation of receptor internalization; thermoregulation; regulation of phosphoprotein phosphatase activity; positive regulation of multicellular organism growth; adult walking behavior; dopamine metabolic process; negative regulation of insulin secretion; dopamine receptor, adenylate cyclase inhibiting pathway; protein localization; negative regulation of blood pressure; phosphatidylinositol metabolic process; response to drug; response to light stimulus; response to inactivity; positive regulation of neuroblast proliferation; cerebral cortex GABAergic interneuron migration; negative regulation of synaptic transmission, glutamatergic; regulation of sodium ion transport; arachidonic acid secretion; regulation of synaptic transmission, GABAergic; positive regulation of growth hormone secretion; G-protein coupled receptor internalization; reduction of cytosolic calcium ion concentration; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; peristalsis; branching morphogenesis of a nerve; negative regulation of circadian sleep/wake cycle, sleep; response to morphine; locomotory behavior; behavioral response to ethanol; orbitofrontal cortex development; negative regulation of cell proliferation; synaptogenesis; behavioral response to cocaine; visual learning; adenohypophysis development; feeding behavior; nerve-nerve synaptic transmission; response to axon injury; circadian regulation of gene expression; negative regulation of dopamine secretion; negative regulation of cell migration; response to iron ion; associative learning; grooming behavior; positive regulation of cytokinesis; negative regulation of protein secretion; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; striatum development; regulation of heart rate; regulation of potassium ion transport; regulation of cAMP metabolic process; negative regulation of adenylate cyclase activity; sensory perception of smell; response to amphetamine; auditory behavior; regulation of dopamine uptake; response to cocaine; cellular calcium ion homeostasis; regulation of systemic arterial blood pressure by neurological process; negative regulation of dopamine receptor signaling pathway; pigmentation; axonogenesis; long-term memory; release of sequestered calcium ion into cytosol; positive regulation of G-protein coupled receptor protein signaling pathway; response to hypoxia; regulation of synapse structural plasticity; dopamine receptor, phospholipase C activating pathway
Disease: Myoclonic Dystonia
Research Articles on D2R
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Product Notes
The Human D2R drd2 (Catalog #AAA9715642) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715642 ELISA Kit recognizes Human D2R. It is sometimes possible for the material contained within the vial of "Dopamine D2 Receptor (D2R), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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