Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Vitamin D2 (VD2) Standard Curve.)
Mouse Vitamin D2 (VD2) ELISA Kit | VD2 elisa kit
Mouse Vitamin D2 (VD2) ELISA Kit
Reactivity
Mouse
Synonyms
Vitamin D2 (VD2); Mouse Vitamin D2 (VD2) ELISA Kit; VD2 elisa kit
Reactivity
Mouse
Specificity
Mouse Vitamin D2 (VD2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse VD2. No significant cross-reactivity or interference between Mouse VD2 and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Vitamin D2 (VD2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Vitamin D2 (VD2) Standard Curve.)
Related Product Information for VD2 elisa kit
Description: This Mouse Vitamin D2 (VD2) ELISA Kit employs a two-site sandwich ELISA to quantitate VD2 in samples. An antibody specific for VD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Ergocalciferol is a provitamin form of vitamin D, also called vitamin D2. Ergocalciferol is created when ultraviolet light activates ergosterol. Ergocalciferol is produced in fungi synthetically through irradiation of ergosterol. Human bioavailability of vitamin D2 from vitamin D2-enhanced button mushrooms via UV-B irradiation is effective in improving vitamin D status and not different from a vitamin D2 supplement. Vitamin D2 from UV-irradiated yeast baked into bread is bioavailable. By visual assessment or using a chromometer, no significant discoloration of irradiated mushrooms, as measured by the degree of "whiteness", was observed. One study found low vitamin D2 levels in patients with Alzheimer's disease, but this observational study did not prove cause or effect related to ergocalciferol and vitamin D2 deficiency in the diet.
Background: Ergocalciferol is a provitamin form of vitamin D, also called vitamin D2. Ergocalciferol is created when ultraviolet light activates ergosterol. Ergocalciferol is produced in fungi synthetically through irradiation of ergosterol. Human bioavailability of vitamin D2 from vitamin D2-enhanced button mushrooms via UV-B irradiation is effective in improving vitamin D status and not different from a vitamin D2 supplement. Vitamin D2 from UV-irradiated yeast baked into bread is bioavailable. By visual assessment or using a chromometer, no significant discoloration of irradiated mushrooms, as measured by the degree of "whiteness", was observed. One study found low vitamin D2 levels in patients with Alzheimer's disease, but this observational study did not prove cause or effect related to ergocalciferol and vitamin D2 deficiency in the diet.
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Product Notes
The Mouse VD2 (Catalog #AAA9716636) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716636 ELISA Kit recognizes Mouse VD2. It is sometimes possible for the material contained within the vial of "Vitamin D2 (VD2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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