Typical Testing Data/Standard Curve (for reference only)
Rat Cholecystokinin (CCK) ELISA Kit | CCK elisa kit
Rat Cholecystokinin (CCK) Mini Samples ELISA Kit
Reactivity
Rat
Applications
ELISA
Synonyms
Cholecystokinin (CCK); Rat Cholecystokinin (CCK) Mini Samples ELISA Kit; CCK-PZ; CCK elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of cholecystokinin. No significant cross-reactivity or interference between cholecystokinin and analogues was observed.
Applicable Applications for CCK elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked Immunosorbent Assay for Antigen Detection.
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
9.88-800pg/mL
Sensitivity
< 3.39pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level cholecystokinin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level cholecystokinin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCK elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of cholecystokinin in rat serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to cholecystokinin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled cholecystokinin and unlabeled cholecystokinin (Standards or samples) with the pre-coated antibody specific to cholecystokinin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of cholecystokinin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of cholecystokinin in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to cholecystokinin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled cholecystokinin and unlabeled cholecystokinin (Standards or samples) with the pre-coated antibody specific to cholecystokinin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of cholecystokinin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of cholecystokinin in the sample.
Product Categories/Family for CCK elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,841 Da
NCBI Official Full Name
cholecystokinin preproprotein
NCBI Official Synonym Full Names
cholecystokinin
NCBI Official Symbol
Cck
NCBI Protein Information
cholecystokinin
UniProt Protein Name
Cholecystokinin
Protein Family
UniProt Gene Name
Cck
UniProt Synonym Gene Names
CCK; CCK39; CCK33; CCK22; CCK12; CCK8
UniProt Entry Name
CCKN_RAT
NCBI Description
brain-gut peptide with hemacrine and autocrine function in the gut [RGD, Feb 2006]
Uniprot Description
Function: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
Subunit structure: Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain
By similarity.
Subcellular location: Secreted.
Tissue specificity: The shortest form (CCK8) is predominantly found in the brain, whereas the larger ones are found in the intestine.
Post-translational modification: The precursor is cleaved by proteases to produce a number of active cholecystokinins.Sulfation of Tyr-97 is essential for receptor activation.
Sequence similarities: Belongs to the gastrin/cholecystokinin family.
Research Articles on CCK
Similar Products
Product Notes
The Rat CCK cck (Catalog #AAA2124070) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2124070 ELISA Kit recognizes Rat CCK. AAA Biotech's Cholecystokinin (CCK) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked Immunosorbent Assay for Antigen Detection. Researchers should empirically determine the suitability of the CCK cck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cholecystokinin (CCK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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