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Typical Testing Data/Standard Curve (for reference only)

Mouse Cholecystokinin (CCK) ELISA Kit | CCK elisa kit

Mouse Cholecystokinin (CCK) ELISA Kit

Reactivity
Mouse
Synonyms
Cholecystokinin (CCK); Mouse Cholecystokinin (CCK) ELISA Kit; CCK-PZ; Cholecystokinin-58 desnonopeptide; CCK elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Cholecystokinin (CCK).
No significant cross-reactivity or interference between Cholecystokinin (CCK) and analogues was observed.
Sequence Length
115
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
12.35-1000pg/mL
Sensitivity
< 4.61pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Cholecystokinin (CCK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Cholecystokinin (CCK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCK elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cholecystokinin (CCK) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cholecystokinin (CCK) and unlabeled Cholecystokinin (CCK) (Standards or samples) with the pre-coated antibody specific to Cholecystokinin (CCK). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cholecystokinin (CCK) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cholecystokinin (CCK) in the sample.
Product Categories/Family for CCK elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
885
UniProt Accession #
Molecular Weight
12,669 Da
NCBI Official Full Name
cholecystokinin
NCBI Official Synonym Full Names
cholecystokinin
NCBI Official Symbol
CCK
NCBI Protein Information
cholecystokinin
UniProt Protein Name
Cholecystokinin
Protein Family
UniProt Gene Name
CCK
UniProt Synonym Gene Names
CCK; CCK58; CCK39; CCK33; CCK25; CCK18; CCK12; CCK8; CCK7; CCK5
UniProt Entry Name
CCKN_HUMAN

NCBI Description

Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

CCK: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. Belongs to the gastrin/cholecystokinin family.

Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted; Hormone

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: axon hillock; extracellular space; axon; dendrite; extracellular region; terminal button; perikaryon

Molecular Function: neuropeptide hormone activity; protein binding; hormone activity

Biological Process: caspase activation; positive regulation of protein oligomerization; release of cytochrome c from mitochondria; positive regulation of peptidyl-tyrosine phosphorylation; behavioral fear response; axonogenesis; positive regulation of mitochondrial depolarization; eating behavior; positive regulation of cell proliferation; protein kinase C activation; neuron migration; regulation of sensory perception of pain; signal transduction; negative regulation of appetite

Research Articles on CCK

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Product Notes

The Mouse CCK cck (Catalog #AAA2024690) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024690 ELISA Kit recognizes Mouse CCK. It is sometimes possible for the material contained within the vial of "Cholecystokinin (CCK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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