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Immunohistochemistry (IHC) (Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Mouse anti-Human PEG10 Monoclonal Antibody

PEG10 (Retrotransposon-derived Protein PEG10, Embryonal Carcinoma Differentiation-regulated Protein, Mammalian Retrotransposon-derived Protein 2, Myelin Expression Factor 3-like Protein 1, MEF3-like Protein 1, Paternally Expressed Gene 10 Protein, Retrotr

Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Ascites
Ascites
Synonyms
PEG10; Monoclonal Antibody; PEG10 (Retrotransposon-derived Protein PEG10; Embryonal Carcinoma Differentiation-regulated Protein; Mammalian Retrotransposon-derived Protein 2; Myelin Expression Factor 3-like Protein 1; MEF3-like Protein 1; Paternally Expressed Gene 10 Protein; Retrotr; Anti -PEG10 (Retrotransposon-derived Protein PEG10; anti-PEG10 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1B1C4
Specificity
Recognizes human PEG10.
Purity/Purification
Ascites
Ascites
Form/Format
Supplied as a liquid, 0.03% sodium azide.
Applicable Applications for anti-PEG10 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 1:200-1:1000
ELISA: 1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:200
Immunogen
Recombinant corresponding to human PEG10.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC) (Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis using PEG10 monoclonal antibody against truncated Trx-PEG10 recombinant protein(1),truncated GST-PEG10 (aa1-120) recombinant protein(2) and full-length PEG10 (aa1-325)-hIgGFc transfected CHO-K1 cell lysate(3). .)

Western Blot (WB) (Western blot analysis using PEG10 monoclonal antibody against truncated Trx-PEG10 recombinant protein(1),truncated GST-PEG10 (aa1-120) recombinant protein(2) and full-length PEG10 (aa1-325)-hIgGFc transfected CHO-K1 cell lysate(3). .)

Immunofluorescence (IF)

(Confocal immunofluorescence analysis of methanol-fixed HepG2 cells using anti-PEG10 monoclonal antioby (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.)

Immunofluorescence (IF) (Confocal immunofluorescence analysis of methanol-fixed HepG2 cells using anti-PEG10 monoclonal antioby (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.)
Related Product Information for anti-PEG10 antibody
Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.
Product Categories/Family for anti-PEG10 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
peg10

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Product Notes

The PEG10 (Catalog #AAA644749) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PEG10 (Retrotransposon-derived Protein PEG10, Embryonal Carcinoma Differentiation-regulated Protein, Mammalian Retrotransposon-derived Protein 2, Myelin Expression Factor 3-like Protein 1, MEF3-like Protein 1, Paternally Expressed Gene 10 Protein, Retrotr reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PEG10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Dilution: Immunofluorescence: 1:200-1:1000 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry (Formalin fixed paraffin embedded): 1:200. Researchers should empirically determine the suitability of the PEG10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEG10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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