Rat Cholecystokinin (CCK) ELISA Kit | CCK elisa kit

Rat Cholecystokinin (CCK) ELISA Kit

Cat. Num. AAA2022187

• Reactivity: Rat
• Synonyms: Cholecystokinin (CCK); Rat Cholecystokinin (CCK) ELISA Kit; CCK-PZ; Cholecystokinin-58 desnonopeptide; CCK elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholecystokinin (CCK).; No significant cross-reactivity or interference between Cholecystokinin (CCK) and analogues was observed.
• Sequence Length: 115

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 9.88-800pg/mL
• Sensitivity: < 4.17pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin (CCK) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin (CCK) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCK elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cholecystokinin (CCK) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cholecystokinin (CCK) and unlabeled Cholecystokinin (CCK) (Standards or samples) with the pre-coated antibody specific to Cholecystokinin (CCK). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cholecystokinin (CCK) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cholecystokinin (CCK) in the sample.

Product Categories/Family for CCK elisa kit

Tumor immunity; Endocrinology; Neuroscience; Hepatology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 179996
• NCBI GeneID: 885
• UniProt Accession #: P06307
• Molecular Weight: 12,669 Da
• NCBI Official Full Name: cholecystokinin
• NCBI Official Synonym Full Names: cholecystokinin
• NCBI Official Symbol: CCK
• NCBI Protein Information: cholecystokinin
• UniProt Protein Name: Cholecystokinin
• Protein Family: Cholecystokinin
• UniProt Gene Name: CCK
• UniProt Synonym Gene Names: CCK; CCK58; CCK39; CCK33; CCK25; CCK18; CCK12; CCK8; CCK7; CCK5
• UniProt Entry Name: CCKN_HUMAN

NCBI Description

Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

CCK: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. Belongs to the gastrin/cholecystokinin family.

Protein type: Secreted, signal peptide; Cell development/differentiation; Hormone; Secreted

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: axon hillock; extracellular space; axon; dendrite; extracellular region; terminal button; perikaryon

Molecular Function: neuropeptide hormone activity; protein binding; hormone activity

Biological Process: caspase activation; positive regulation of protein oligomerization; positive regulation of peptidyl-tyrosine phosphorylation; release of cytochrome c from mitochondria; behavioral fear response; positive regulation of mitochondrial depolarization; axonogenesis; eating behavior; positive regulation of cell proliferation; protein kinase C activation; neuron migration; regulation of sensory perception of pain; signal transduction; negative regulation of appetite