Rat Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) ELISA Kit | BNIP3 elisa kit

Rat Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) ELISA Kit

Cat. Num. AAA2024250

• Gene Names: BNIP3; NIP3
• Reactivity: Rat
• Synonyms: Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3); Rat Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) ELISA Kit; NIP3; BNIP3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of BNIP3. No significant cross-reactivity or interference between BNIP3 and analogues was observed.
• Sequence Length: 194

• Samples: Rat tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BNIP3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BNIP3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BNIP3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BNIP3 in rat tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to BNIP3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to BNIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BNIP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BNIP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BNIP3 elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 119569528
• NCBI GeneID: 664
• UniProt Accession #: Q12983; O14620; Q96GP0
• Molecular Weight: 21,541 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19kDa interacting protein 3
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B 19kDa interacting protein 3
• NCBI Official Symbol: BNIP3
• NCBI Official Synonym Symbols: NIP3
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Protein Family: BCL2/adenovirus E1B 19 kDa protein-interacting protein
• UniProt Gene Name: BNIP3
• UniProt Synonym Gene Names: NIP3
• UniProt Entry Name: BNIP3_HUMAN

NCBI Description

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]

Uniprot Description

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; mitochondrial membrane; dendrite; cytoplasm; nuclear envelope; nucleus; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; GTPase binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: cell death; positive regulation of protein complex disassembly; induction of apoptosis by granzyme; viral reproduction; positive regulation of apoptosis; apoptosis; positive regulation of programmed cell death; neuron apoptosis; response to hyperoxia; autophagic cell death; brown fat cell differentiation; response to hypoxia; mitochondrial fragmentation during apoptosis; regulation of mitochondrial membrane permeability; negative regulation of membrane potential; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis