Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 Recombinant Protein | BNIP3 recombinant protein

Recombinant Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3)

Cat. Num. AAA2034287

• Gene Names: BNIP3; NIP3
• Reactivity: Rat
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Bcl2/Adenovirus E1B 19kDa Interacting Protein 3; Recombinant Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3); NIP3; BNIP3 recombinant protein

• Host: E Coli
• Reactivity: Rat
• Purity/Purification: >90%
• Sequence Length: 259

• Applicable Applications for BNIP3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Fragment: Gln3~Ala134
• Tag: Prokaryotic expression
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 734703979
• NCBI GeneID: 664
• NCBI Accession #: NP_004043.3
• NCBI GenBank Nucleotide #: NM_004052.3
• Molecular Weight: 45.0kDa
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B 19kDa interacting protein 3
• NCBI Official Symbol: BNIP3
• NCBI Official Synonym Symbols: NIP3
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Protein Family: BCL2/adenovirus E1B 19 kDa protein-interacting protein
• UniProt Gene Name: BNIP3
• UniProt Synonym Gene Names: NIP3
• UniProt Entry Name: BNIP3_HUMAN

NCBI Description

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]

Uniprot Description

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: cytoplasm; dendrite; endoplasmic reticulum; integral to mitochondrial outer membrane; mitochondrial membrane; mitochondrial outer membrane; mitochondrion; nuclear envelope; nucleoplasm; nucleus

Molecular Function: GTPase binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: apoptosis; autophagic cell death; brown fat cell differentiation; cell death; defense response to virus; induction of apoptosis by granzyme; mitochondrial fragmentation during apoptosis; mitochondrion degradation; negative regulation of apoptosis; negative regulation of membrane potential; neuron apoptosis; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of macroautophagy; positive regulation of programmed cell death; positive regulation of protein complex disassembly; regulation of mitochondrial membrane permeability; response to hyperoxia; response to hypoxia; viral reproduction