Rat Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) CLIA Kit | BNIP3 clia kit

Rat Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) CLIA Kit

Cat. Num. AAA2000625

• Gene Names: BNIP3; NIP3
• Reactivity: Rat
• Synonyms: Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3); Rat Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) CLIA Kit; NIP3; BNIP3 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3).; No significant cross-reactivity or interference between Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) and analogues was observed.
• Sequence Length: 259

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 54.9-40,000pg/mL
• Sensitivity: < 22.7pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BNIP3 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) level in the sample or standard.;

Product Categories/Family for BNIP3 clia kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 119569528
• NCBI GeneID: 664
• UniProt Accession #: Q12983; O14620; Q96GP0
• Molecular Weight: 21,541 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19kDa interacting protein 3
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B 19kDa interacting protein 3
• NCBI Official Symbol: BNIP3
• NCBI Official Synonym Symbols: NIP3
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Protein Family: BCL2/adenovirus E1B 19 kDa protein-interacting protein
• UniProt Gene Name: BNIP3
• UniProt Synonym Gene Names: NIP3
• UniProt Entry Name: BNIP3_HUMAN

NCBI Description

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]

Uniprot Description

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytoplasm; dendrite; mitochondrial membrane; nuclear envelope; nucleus; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; GTPase binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: cell death; positive regulation of protein complex disassembly; induction of apoptosis by granzyme; viral reproduction; apoptosis; positive regulation of apoptosis; positive regulation of programmed cell death; neuron apoptosis; response to hyperoxia; autophagic cell death; response to hypoxia; brown fat cell differentiation; regulation of mitochondrial membrane permeability; mitochondrial fragmentation during apoptosis; negative regulation of membrane potential; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis