Rat Basigin (BSG) ELISA Kit | BSG elisa kit

Rat Basigin (BSG) ELISA Kit

Cat. Num. AAA9712531

• Gene Names: BSG; CD147
• Reactivity: Rat
• Synonyms: Basigin (BSG); Rat Basigin (BSG) ELISA Kit; BSG; 5F7; CD147; EMMPRIN; M6; OK; TCSF; CD147 antigen; M6 leukocyte activation antigen; OK blood group antigen; basigin; collagenase stimulatory factor; extracellular matrix metalloproteinase inducer; BSG elisa kit

• Reactivity: Rat
• Specificity: Rat Basigin (BSG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat BSG. No significant cross-reactivity or interference between Rat BSG and analogues was observed.
• Sequence Length: 270

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Basigin (BSG) Standard Curve.)

Related Product Information for BSG elisa kit

Description: This Rat Basigin (BSG) ELISA Kit employs a two-site sandwich ELISA to quantitate BSG in samples. An antibody specific for BSG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BSG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BSG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BSG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Basigin is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition.It has a variety of functions. In addition to its metalloproteinase-inducing ability, basigin also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes.Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin proteins Cyp-A and CyP-B and certain integrins.

NCBI and Uniprot Product Information

• NCBI GI #: 162287046
• NCBI GeneID: 100009229
• NCBI Accession #: NP_001075843.1
• NCBI GenBank Nucleotide #: NP_001075843.1
• UniProt Accession #: Q28740
• NCBI Official Full Name: basigin
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: CD147
• NCBI Protein Information: basigin
• UniProt Protein Name: Basigin
• Protein Family: Basigin
• UniProt Gene Name: BSG

Uniprot Description

Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3, SLC16A8 and SLC16A11 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.