Human Emmprin ELISA Kit | BSG elisa kit

Human Emmprin PicoKine ELISA Kit

Cat. Num. AAA175872

• Gene Names: BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
• Reactivity: Human
• Synonyms: Emmprin; Human Emmprin PicoKine ELISA Kit; Basigin; basigin (Ok blood group); 5F7; BASI_HUMAN; Bsg; CD147; Collagenase stimulatory factor; EMMPRIN; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; OK blood group antigen; TCSF; Tumor cell-derived collagenase stimulatory factor; BSG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Emmprin
• Sequence Length: 385

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human Emmprin PicoKine ELISA Kit standard curve )

Related Product Information for BSG elisa kit

Principle of the assay: human Emmprin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Emmprin has been precoated onto 96-well plates. Standards(NSO, T25-H205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Emmprin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Emmprin amount of sample captured in plate.
Background: Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147 (cluster of differentiation 147).[1][2] It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition (Miyauchi et al., 1991; Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.[3][4][5] It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 51704273
• NCBI GeneID: 682
• UniProt Accession #: P35613; Q7Z796; Q8IZL7; A6NJW1; D3YLG5
• Molecular Weight: 22,764 Da
• NCBI Official Full Name: Basigin
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: M6; OK; 5F7; TCSF; CD147; EMMPRIN
• NCBI Protein Information: basigin; CD147 antigen; OK blood group antigen; collagenase stimulatory factor; leukocyte activation antigen M6; extracellular matrix metalloproteinase inducer; tumor cell-derived collagenase stimulatory factor
• UniProt Protein Name: Basigin
• Protein Family: Basigin
• UniProt Gene Name: BSG
• UniProt Synonym Gene Names: EMMPRIN; TCSF
• UniProt Entry Name: BASI_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BSG: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Enriched on the surface of tumor cells. Up-regulated in gliomas. Its expression levels correlate with malignant potential of the tumor. Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; focal adhesion; membrane; mitochondrion; integral to plasma membrane; melanosome; plasma membrane; sarcolemma; lipid raft; acrosomal membrane

Molecular Function: mannose binding; protein binding

Biological Process: response to mercury ion; cellular metabolic process; response to peptide hormone stimulus; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell surface receptor linked signal transduction; response to cAMP; decidualization; blood coagulation; pyruvate metabolic process; leukocyte migration; embryo implantation; odontogenesis of dentine-containing teeth

Disease: Blood Group--ok