Typical Testing Data/Standard Curve (for reference only)
Human Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit | TOMM22 elisa kit
Human Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TOMM22 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TOMM22 antibody and an TOMM22-HRP conjugate. The assay sample and buffer are incubated together with TOMM22-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TOMM22 concentration since TOMM22 from samples and TOMM22-HRP conjugate compete for the anti-TOMM22 antibody binding site. Since the number of sites is limited, as more sites are occupied by TOMM22 from the sample, fewer sites are left to bind TOMM22-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TOMM22 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion. [provided by RefSeq, Jul 2008]
Uniprot Description
TOMM22: Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Belongs to the Tom22 family.
Protein type: Mitochondrial; Membrane protein, integral
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: membrane; mitochondrial outer membrane translocase complex; mitochondrion; mitochondrial inner membrane; integral to membrane
Molecular Function: protein binding; protein transmembrane transporter activity
Biological Process: cellular protein metabolic process; protein import into mitochondrial outer membrane; protein targeting to mitochondrion
Research Articles on TOMM22
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Product Notes
The Human TOMM22 tomm22 (Catalog #AAA7226957) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7226957 ELISA Kit recognizes Human TOMM22. It is sometimes possible for the material contained within the vial of "Mitochondrial import receptor subunit TOM22 homolog (TOMM22), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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