Rat arginine vasopressin, AVP ELISA Kit | AVP elisa kit

Rat arginine vasopressin, AVP ELISA Kit

Cat. Num. AAA731489

• Reactivity: Rat
• Synonyms: arginine vasopressin; AVP; Rat arginine vasopressin; AVP ELISA Kit; AVP ELISA Kit (Rat); AVP elisa kit

• Reactivity: Rat

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AVP elisa kit

Intended Uses: This AVP ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AVP in the sample, this AVP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AVP concentration. The concentration of AVP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This AVP enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for AVP. Standards or samples are then added to the microtiter plate wells and AVP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AVP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AVP are added to each well to "sandwich" the AVP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain AVP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for AVP elisa kit

Rat ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 10334810
• NCBI Official Full Name: arginine vasopressin
• Protein Family: Neurophysin

NCBI Description

This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]

Uniprot Description

AVP: Neurophysin 2 specifically binds vasopressin. Defects in AVP are the cause of diabetes insipidus, neurohypophyseal (NDI). A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopression deficiency during childhood. Belongs to the vasopressin/oxytocin family.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular space; dendrite; extracellular region; cytosol; secretory granule

Molecular Function: neuropeptide hormone activity; signal transducer activity; V1A vasopressin receptor binding; caspase inhibitor activity; neurohypophyseal hormone activity; V1B vasopressin receptor binding; receptor binding; protein kinase activity

Biological Process: circadian rhythm; response to nicotine; water transport; positive regulation of glutamate secretion; positive regulation of cellular pH reduction; positive regulation of systemic arterial blood pressure; locomotory behavior; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; elevation of cytosolic calcium ion concentration; cell-cell signaling; hyperosmotic salinity response; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; transmembrane transport; penile erection; positive regulation of prostaglandin biosynthetic process; grooming behavior; vasoconstriction; sodium-independent organic anion transport; generation of precursor metabolites and energy; maternal behavior; social behavior; response to testosterone stimulus; positive regulation of peptidyl-serine phosphorylation; positive regulation of cell growth; negative regulation of female receptivity; response to ethanol; negative regulation of transmission of nerve impulse; positive regulation of vasoconstriction; renal water homeostasis; negative regulation of apoptosis

Disease: Diabetes Insipidus, Neurohypophyseal