Human arginine vasopressin, AVP ELISA Kit | AVP elisa kit

Human arginine vasopressin, AVP ELISA Kit

Cat. Num. AAA733623

• Reactivity: Human
• Synonyms: arginine vasopressin; AVP; Human arginine vasopressin; AVP ELISA Kit; AVP ELISA Kit (Human); AVP elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AVP elisa kit

Intended Uses: This ATXN3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ATXN3. This ELISA kit for research use only!

Principle of the Assay: ATXN3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATXN3 antibody and an ATXN3-HRP conjugate. The assay sample and buffer are incubated together with ATXN3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATXN3 concentration since ATXN3 from samples and ATXN3-HRP conjugate compete for the anti-ATXN3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATXN3 from the sample, fewer sites are left to bind ATXN3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATXN3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AVP elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 10334810
• NCBI Official Full Name: arginine vasopressin
• Protein Family: Neurophysin

NCBI Description

This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]

Uniprot Description

AVP: Neurophysin 2 specifically binds vasopressin. Defects in AVP are the cause of diabetes insipidus, neurohypophyseal (NDI). A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopression deficiency during childhood. Belongs to the vasopressin/oxytocin family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular space; dendrite; extracellular region; cytosol; secretory granule

Molecular Function: neuropeptide hormone activity; signal transducer activity; V1A vasopressin receptor binding; caspase inhibitor activity; neurohypophyseal hormone activity; V1B vasopressin receptor binding; receptor binding; protein kinase activity

Biological Process: response to nicotine; circadian rhythm; water transport; positive regulation of cellular pH reduction; positive regulation of glutamate secretion; locomotory behavior; positive regulation of systemic arterial blood pressure; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; elevation of cytosolic calcium ion concentration; cell-cell signaling; hyperosmotic salinity response; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; transmembrane transport; positive regulation of prostaglandin biosynthetic process; penile erection; grooming behavior; vasoconstriction; generation of precursor metabolites and energy; sodium-independent organic anion transport; maternal behavior; response to testosterone stimulus; social behavior; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; negative regulation of female receptivity; response to ethanol; negative regulation of transmission of nerve impulse; positive regulation of vasoconstriction; renal water homeostasis; negative regulation of apoptosis

Disease: Diabetes Insipidus, Neurohypophyseal