Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Adrenomedullin 2 (ADM2) Standard Curve.)
Rat Adrenomedullin 2 (ADM2) ELISA Kit | ADM2 elisa kit
Rat Adrenomedullin 2 (ADM2) ELISA Kit
Gene Names
Adm2; Imd
Reactivity
Rat
Synonyms
Adrenomedullin 2 (ADM2); Rat Adrenomedullin 2 (ADM2) ELISA Kit; ADM2; RP4-579N16.10; AM2; FLJ21135; dJ579N16.4; OTTHUMP00000196596; intermedin; ADM2 elisa kit
Reactivity
Rat
Specificity
Rat Adrenomedullin 2 (ADM2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ADM2. No significant cross-reactivity or interference between Rat ADM2 and analogues was observed.
Sequence Length
146
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Adrenomedullin 2 (ADM2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Adrenomedullin 2 (ADM2) Standard Curve.)
Related Product Information for ADM2 elisa kit
Description: This Rat Adrenomedullin 2 (ADM2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADM2 in samples. An antibody specific for ADM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ADM2 belongs to a family of calcitonin related peptide hormones important for regulating diverse physiologic functions and the chemical composition of fluids and tissues. ADM2 contains a dibasic cleavage site, followed by a 47-amino acid mature peptide hormone sequence and an alpha-amidation donor glycine at the C terminus. Immunohistochemical analysis of several mouse tissues detected Adm2 in pituitary and stomach. PCR analysis of a human gastrointestinal panel detected ADM2 in almost all regions examined. Immunohistochemical staining showed ADM2 in the muscularis mucosae layer of stomach. Western blot analysis of transfected embryonic kidney cells found mature ADM2 secreted into the culture medium; the apparent molecular mass was about 5.0 kD.
Background: ADM2 belongs to a family of calcitonin related peptide hormones important for regulating diverse physiologic functions and the chemical composition of fluids and tissues. ADM2 contains a dibasic cleavage site, followed by a 47-amino acid mature peptide hormone sequence and an alpha-amidation donor glycine at the C terminus. Immunohistochemical analysis of several mouse tissues detected Adm2 in pituitary and stomach. PCR analysis of a human gastrointestinal panel detected ADM2 in almost all regions examined. Immunohistochemical staining showed ADM2 in the muscularis mucosae layer of stomach. Western blot analysis of transfected embryonic kidney cells found mature ADM2 secreted into the culture medium; the apparent molecular mass was about 5.0 kD.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein ADM2
NCBI Official Synonym Full Names
adrenomedullin 2
NCBI Official Symbol
Adm2
NCBI Official Synonym Symbols
Imd
NCBI Protein Information
protein ADM2
UniProt Protein Name
ADM2
Protein Family
UniProt Gene Name
Adm2
UniProt Synonym Gene Names
Am2; AM2; IMDL; IMDS
UniProt Entry Name
ADM2_RAT
NCBI Description
acts via calcitonin receptor-like receptor (CRLR) and receptor activity-modifying protein (RAMP) protein complexes to induce cAMP production; decreases blood pressure; suppresses gastric emptying activity and food intake [RGD, Feb 2006]
Uniprot Description
Function: IMDL and IMDS may play a role as physiological regulators of gastrointestinal, cardiovascular bioactivities mediated by the CALCRL/RAMPs receptor complexes. Activates the cAMP-dependent pathway.1 PublicationManual assertion based on experiment in:Ref.4
Research Articles on ADM2
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Product Notes
The Rat ADM2 adm2 (Catalog #AAA9711966) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711966 ELISA Kit recognizes Rat ADM2. It is sometimes possible for the material contained within the vial of "Adrenomedullin 2 (ADM2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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