Human Adrenomedullin 2 (ADM2) ELISA Kit | ADM2 elisa kit

Human Adrenomedullin 2 (ADM2) ELISA Kit

Cat. Num. AAA455512

• Gene Names: ADM2; AM2; dJ579N16.4
• Reactivity: Human
• Synonyms: Adrenomedullin 2 (ADM2); Human Adrenomedullin 2 (ADM2) ELISA Kit; AM2 IMDL IMDS Intermedin; ADM2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADM2. No significant cross-reactivity or interference between ADM2 and analogues was observed.
• Sequence Length: 148

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.214ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADM2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADM2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADM2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADM2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 359718926
• NCBI GeneID: 79924
• NCBI Accession #: NP_001240774.1
• NCBI GenBank Nucleotide #: NM_001253845.1
• UniProt Accession #: Q7Z4H4; Q3LFQ0
• Molecular Weight: 15,865 Da
• NCBI Official Full Name: ADM2 preproprotein
• NCBI Official Synonym Full Names: adrenomedullin 2
• NCBI Official Symbol: ADM2
• NCBI Official Synonym Symbols: AM2; dJ579N16.4
• NCBI Protein Information: ADM2
• UniProt Protein Name: ADM2
• Protein Family: ADM
• UniProt Gene Name: ADM2
• UniProt Synonym Gene Names: AM2; AM2; IMDL; IMDS

NCBI Description

This gene encodes a member of the calcitonin gene-related peptide (CGRP)/calcitonin family of hormones that play a role in the regulation of cardiovascular homeostasis, prolactin release, anti-diuresis, anti-natriuresis, and regulation of food and water intake. The encoded protein is proteolytically processed to generate one or more biologically active peptides. [provided by RefSeq, Jul 2015]

Uniprot Description

IMDL and IMDS may play a role as physiological regulators of gastrointestinal, cardiovascular bioactivities mediated by the CALCRL/RAMPs receptor complexes. Activates the cAMP-dependent pathway.