Bcl-XL Active Protein | BCL2L1 active protein

Bcl-XL Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120279

• Gene Names: BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Bcl-XL; Bcl-XL Protein; Human; Recombinant (His Tag); Human BCL2L1/Bcl-XL Protein (His Tag); Bcl-X Protein; bcl-xL Protein; BCL-XL/S Protein; bcl-xS Protein; BCL2L Protein; BCLX Protein; BCLXL Protein; BCLXS Protein; PPP1R52 Protein; BCL2-like 1; BCL2L1 active protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 5mM Tris, 1% Glycerol, pH8. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg212

• Species: Human
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human BID at 10ug/mL (100uL/well) can bind biotinylated human BCL2L1. The EC50 of biotinylated human BCL2L1 is 7.1ng/mL.; 3. Immobilized mouse BID at 10ug/mL (100uL/well) can bind biotinylated human BCL2L1. The EC50 of biotinylated human BCL2L1 is 7.01ng/mL.
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human BCL2L1 (NP_612815.1)(Met1-Arg212) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for BCL2L1 active protein

Background: B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic alpha-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices alphal and alpha2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is characterized as an important factor in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca²⁺ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2/Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Product Categories/Family for BCL2L1 active protein

Death Receptor Signaling

References

Vander Heiden MG, et al. (1997) Bcl-xL Regulates the Membrane Potential and Volume Homeostasis of Mitochondria. Cell. 91 (5): 627-37.Muchmore SW, et al. (1996) X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature. 381: 335-341.SharoffEH, et al. (2007) Bcl-2 family members regulate anoxia-induced cell death. Antioxid Redox Signal. 9 (9) :1405-9.Zhou F, et al. (2011) Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis. FEBS J. 278 (3): 403-13.

NCBI and Uniprot Product Information

• NCBI GI #: 20336335
• NCBI GeneID: 598
• NCBI Accession #: NP_612815.1
• NCBI GenBank Nucleotide #: NM_138578.2
• UniProt Accession #: Q07817; Q5CZ89; Q5TE65; Q92976; E1P5L6
• Molecular Weight: 18,894 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform Bcl-X(L)
• NCBI Official Synonym Full Names: BCL2 like 1
• NCBI Official Symbol: BCL2L1
• NCBI Official Synonym Symbols: BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
• NCBI Protein Information: bcl-2-like protein 1
• UniProt Protein Name: Bcl-2-like protein 1
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L1
• UniProt Synonym Gene Names: BCL2L; BCLX; Bcl2-L-1
• UniProt Entry Name: B2CL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]

Uniprot Description

Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Mitochondrial; Apoptosis; Membrane protein, integral; Autophagy

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: centrosome; nuclear membrane; mitochondrion; synaptic vesicle membrane; integral to membrane; cytosol; mitochondrial outer membrane; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; nucleolus; cell junction; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; BH3 domain binding; protein kinase binding

Biological Process: negative regulation of autophagy; apoptosis; cytokinesis; cellular process regulating host cell cycle in response to virus; germ cell development; apoptotic mitochondrial changes; regulation of mitochondrial membrane potential; ovarian follicle development; positive regulation of cell proliferation; negative regulation of neuron apoptosis; release of cytochrome c from mitochondria; in utero embryonic development; mitotic cell cycle checkpoint; male gonad development; suppression by virus of host apoptosis; endocytosis; cell proliferation; neuron apoptosis; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; innate immune response; response to cycloheximide; spermatogenesis; regulation of mitochondrial membrane permeability; growth; negative regulation of apoptosis