Western Blot (WB)
(DNAJB12 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the DNAJB12 antibody detected the DNAJB12 protein (arrow).)
Rabbit anti-Human DNAJB12 Polyclonal Antibody | anti-DNAJB12 antibody
DNAJB12, NT (DNAJB12, DnaJ homolog subfamily B member 12) (AP)
Gene Names
DNAJB12; DJ10
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DNAJB12; Polyclonal Antibody; NT (DNAJB12; DnaJ homolog subfamily B member 12) (AP); DnaJ homolog subfamily B member 12; anti-DNAJB12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-DNAJB12 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
DNAJB12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human DNAJB12.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(DNAJB12 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the DNAJB12 antibody detected the DNAJB12 protein (arrow).)
Western Blot (WB)
(DNAJB12 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the DNAJB12 antibody detected the DNAJB12 protein (arrow).)
Product Categories/Family for anti-DNAJB12 antibody
References
Lamesch, P., et al. Genomics 89(3):307-315(2007). Ohtsuka, K., et al. Cell Stress Chaperones 5(2):98-112(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,819 Da
NCBI Official Full Name
dnaJ homolog subfamily B member 12
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily B, member 12
NCBI Official Symbol
DNAJB12
NCBI Official Synonym Symbols
DJ10
NCBI Protein Information
dnaJ homolog subfamily B member 12
UniProt Protein Name
DnaJ homolog subfamily B member 12
Protein Family
UniProt Gene Name
DNAJB12
UniProt Entry Name
DJB12_HUMAN
NCBI Description
DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008]
Research Articles on DNAJB12
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Product Notes
The DNAJB12 dnajb12 (Catalog #AAA6292652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJB12, NT (DNAJB12, DnaJ homolog subfamily B member 12) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJB12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DNAJB12 dnajb12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJB12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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