Human Adrenomedullin (ADM) ELISA Kit | ADM elisa kit

Human Adrenomedullin (ADM) ELISA Kit

Cat. Num. AAA9714607

• Gene Names: ADM; AM; PAMP
• Reactivity: Human
• Synonyms: Adrenomedullin (ADM); Human Adrenomedullin (ADM) ELISA Kit; ADM; AM; preproadrenomedullin; ADM elisa kit

• Reactivity: Human
• Specificity: Human Adrenomedullin (ADM) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADM. No significant cross-reactivity or interference between Human ADM and analogues was observed.
• Sequence Length: 185

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Adrenomedullin (ADM) Standard Curve.)

Related Product Information for ADM elisa kit

Description: This Human Adrenomedullin (ADM) ELISA Kit employs a two-site sandwich ELISA to quantitate ADM in samples. An antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Adrenomedullin (ADM) is a peptide associated with pheochromocytoma. Adrenomedullin (AM) is a ubiquitously expressed peptide initially isolated from phaechromyctoma. Adrenomedullin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. The human AM gene is localized to a single locus on Chromosome 11 with 4 exons and 3 introns.

NCBI and Uniprot Product Information

• NCBI GI #: 765330
• NCBI GeneID: 133
• UniProt Accession #: P35318
• NCBI Official Full Name: adrenomedullin
• NCBI Official Synonym Full Names: adrenomedullin
• NCBI Official Symbol: ADM
• NCBI Official Synonym Symbols: AM; PAMP
• NCBI Protein Information: ADM
• UniProt Protein Name: ADM
• Protein Family: ADM
• UniProt Gene Name: ADM
• UniProt Synonym Gene Names: AM; AM; PAMP; ProAM-N20
• UniProt Entry Name: ADML_HUMAN

NCBI Description

The protein encoded by this gene is a preprohormone which is cleaved to form two biologically active peptides, adrenomedullin and proadrenomedullin N-terminal 20 peptide. Adrenomedullin is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. [provided by RefSeq, Aug 2014]

Uniprot Description

ADM: AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. Belongs to the adrenomedullin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; adrenomedullin receptor binding; hormone activity; receptor binding

Biological Process: developmental growth; positive regulation of apoptosis; blood circulation; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; female pregnancy; signal transduction; positive regulation of vasodilation; response to insulin stimulus; negative regulation of cell proliferation; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; cell-cell signaling; receptor internalization; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; response to wounding; vasculogenesis; regulation of the force of heart contraction; aging; hormone secretion; organ regeneration; progesterone biosynthetic process; androgen metabolic process; neurite regeneration; positive regulation of heart rate; negative regulation of vasoconstriction; odontogenesis of dentine-containing teeth; response to starvation; positive regulation of angiogenesis; G-protein coupled receptor internalization; neural tube closure; response to hypoxia; negative regulation of vascular permeability; cAMP biosynthetic process; response to cold