V (D)J recombination-activating protein 1 Recombinant Protein | RAG1 recombinant protein

Recombinant Human V (D)J recombination-activating protein 1 , partial

• Gene Names: RAG1; RAG-1; RNF74
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: V (D)J recombination-activating protein 1; Recombinant Human V (D)J recombination-activating protein 1; partial; RAG1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 931

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for RAG1 recombinant protein

This protein is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 4557841
• NCBI GeneID: 5896
• NCBI Accession #: NP_000439.1
• NCBI GenBank Nucleotide #: NM_000448.2
• UniProt Accession #: P15918; Q8IY72; Q8NER2; E9PPC4
• Molecular Weight: 106,179 Da
• NCBI Official Full Name: V(D)J recombination-activating protein 1
• NCBI Official Synonym Full Names: recombination activating 1
• NCBI Official Symbol: RAG1
• NCBI Official Synonym Symbols: RAG-1; RNF74
• NCBI Protein Information: V(D)J recombination-activating protein 1
• UniProt Protein Name: V(D)J recombination-activating protein 1
• Protein Family: V(D)J recombination-activating protein
• UniProt Gene Name: RAG1
• UniProt Synonym Gene Names: RNF74; RAG-1

NCBI Description

The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1 ().