Human V(D)J recombination-activating protein 1 ELISA Kit | RAG1 elisa kit

Human V(D)J recombination-activating protein 1 ELISA Kit

Cat. Num. AAA288537

• Gene Names: RAG1; RAG-1; RNF74
• Reactivity: Human
• Synonyms: V(D)J recombination-activating protein 1; Human V(D)J recombination-activating protein 1 ELISA Kit; RAG1 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human RAG-1. No significant cross-reactivity or interference was observed.
• Sequence Length: 1043

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.098ng/mL
• Intra-Assay CV: <=4.1%
• Inter-Assay CV: <=7.3%
• Recovery: 101%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAG1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAG-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RAG-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RAG-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RAG-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557841
• NCBI GeneID: 5896
• NCBI Accession #: NP_000439.1
• NCBI GenBank Nucleotide #: NM_000448.2
• UniProt Accession #: P15918; Q8IY72; Q8NER2; E9PPC4
• Molecular Weight: 106,179 Da
• NCBI Official Full Name: V(D)J recombination-activating protein 1
• NCBI Official Synonym Full Names: recombination activating gene 1
• NCBI Official Symbol: RAG1
• NCBI Official Synonym Symbols: RAG-1; RNF74
• NCBI Protein Information: V(D)J recombination-activating protein 1
• UniProt Protein Name: V(D)J recombination-activating protein 1
• Protein Family: V(D)J recombination-activating protein
• UniProt Gene Name: RAG1
• UniProt Synonym Gene Names: RNF74; RAG-1
• UniProt Entry Name: RAG1_HUMAN

NCBI Description

The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

RAG1: Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'- hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'- phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1. Homodimer. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Maturing lymphoid cells. Belongs to the RAG1 family.

Protein type: EC 3.1.-.-; EC 6.3.2.-; DNA-binding; Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: nucleoplasm; nucleus

Molecular Function: acid-amino acid ligase activity; DNA binding; endonuclease activity; histone binding; metal ion binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: adaptive immune response; B cell differentiation; DNA recombination; histone monoubiquitination; immune response; negative regulation of caspase activity; positive regulation of T cell differentiation; pre-B cell allelic exclusion; protein autoubiquitination; T cell differentiation in the thymus; T cell homeostasis; thymus development; V(D)J recombination

Disease: Alpha/beta T-cell Lymphopenia With Gamma/delta T-cell Expansion, Severe Cytomegalovirus Infection, And Autoimmunity; Combined Cellular And Humoral Immune Defects With Granulomas; Omenn Syndrome; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-positive