Rabbit anti-Human HSD11B2 Polyclonal Antibody | anti-HSD11B2 antibody

HSD11B2, ID (HSD11B2, HSD11K, Corticosteroid 11-beta-dehydrogenase isozyme 2, 11-beta-hydroxysteroid dehydrogenase type 2, 11-beta-hydroxysteroid dehydrogenase type II, NAD-dependent 11-beta-hydroxysteroid dehydrogenase) (Azide free) (HRP)

Cat. Num. AAA6308445

• Gene Names: HSD11B2; AME; AME1; HSD2; HSD11K; SDR9C3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HSD11B2; Polyclonal Antibody; ID (HSD11B2; HSD11K; Corticosteroid 11-beta-dehydrogenase isozyme 2; 11-beta-hydroxysteroid dehydrogenase type 2; 11-beta-hydroxysteroid dehydrogenase type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase) (Azide free) (HRP); NAD-dependent 11-beta-hydroxysteroid dehydrogenase; anti-HSD11B2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HSD11B2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HSD11B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284~314 amino acids from the Central region of human HSD11B2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HSD11B2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). HSD11B2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(HSD11B2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSD11B2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HSD11B2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HSD11B2 antibody

There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.

Product Categories/Family for anti-HSD11B2 antibody

Antibodies; Enzymes; Dehydrogenase

References

Li, J., et al. Breast Cancer Res. 12 (2), R19 (2010). Ni, X.T., et al. Placenta 30(12):1023-1028(2009). Mericq, V., et al. Eur. J. Endocrinol. 161(3):419-425(2009). Stark, M.J., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 297 (2), R510-R514 (2009). Lepenies, J., et al. Clin. Exp. Hypertens. 31(4):376-379(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 119392083
• NCBI GeneID: 3291
• NCBI Accession #: NP_000187.3
• NCBI GenBank Nucleotide #: NM_000196.3
• UniProt Accession #: P80365; Q13194; Q6P2G9; Q8N439; Q96QN8; Q9UC50; Q9UC51; Q9UCW5; Q9UCW6; Q9UCW7; A7LB28; C5HTY7
• Molecular Weight: 44,127 Da
• NCBI Official Full Name: corticosteroid 11-beta-dehydrogenase isozyme 2
• NCBI Official Synonym Full Names: hydroxysteroid (11-beta) dehydrogenase 2
• NCBI Official Symbol: HSD11B2
• NCBI Official Synonym Symbols: AME; AME1; HSD2; HSD11K; SDR9C3
• NCBI Protein Information: corticosteroid 11-beta-dehydrogenase isozyme 2
• UniProt Protein Name: Corticosteroid 11-beta-dehydrogenase isozyme 2
• Protein Family: Corticosteroid 11-beta-dehydrogenase isozyme
• UniProt Gene Name: HSD11B2
• UniProt Synonym Gene Names: 11-DH2; 11-beta-HSD2; 11-HSD type II; 11-beta-HSD type II; 11-beta-HSD
• UniProt Entry Name: DHI2_HUMAN

NCBI Description

There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010]

Uniprot Description

HSD11B2: Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME). An autosomal recessive form of low-renin hypertension. It is usually diagnosed within the first years of life and is characterized by polyuria and polydipsia, failure to thrive, hypernatremia, severe hypertension with low renin and aldosterone levels, profound hypokalemia with metabolic alkalosis, and most often nephrocalcinosis. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: EC 1.1.1.-; Lipid Metabolism - androgen and estrogen; Oxidoreductase; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: endoplasmic reticulum membrane

Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity; NAD binding; steroid binding

Biological Process: aldosterone mediated regulation of blood volume; female pregnancy; glucocorticoid biosynthetic process; response to drug; response to food; response to glucocorticoid stimulus; response to hypoxia; response to insulin stimulus

Disease: Apparent Mineralocorticoid Excess