Rabbit anti-Human IL1R2 Polyclonal Antibody | anti-IL1R2 antibody

IL1R2 (Interleukin 1 Receptor Type II, Interleukin-1 Receptor beta, IL1RB, CD121b, IL1R2c, CDw121b, IL-1R-2, IL-1RT2, IL-1RT-2) (FITC)

Cat. Num. AAA6422473

• Gene Names: IL1R2; IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: IL1R2; Polyclonal Antibody; IL1R2 (Interleukin 1 Receptor Type II; Interleukin-1 Receptor beta; IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2) (FITC); anti-IL1R2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IL1R2.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-IL1R2 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human IL1R2.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using IL1R2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid cancer using IL1R2 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat lung using IL1R2 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using IL1R2 antibody at dilution of 1:200 (200x lens).)

Product Categories/Family for anti-IL1R2 antibody

Antibodies; Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 387527984
• NCBI GeneID: 7850
• NCBI Accession #: NP_001248348.1
• NCBI GenBank Nucleotide #: NM_001261419.1
• UniProt Accession #: P27930; Q6LCE6; Q9UE68; D3DVJ5
• Molecular Weight: 45,421 Da
• NCBI Official Full Name: interleukin-1 receptor type 2 isoform 2
• NCBI Official Synonym Full Names: interleukin 1 receptor, type II
• NCBI Official Symbol: IL1R2
• NCBI Official Synonym Symbols: IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2
• NCBI Protein Information: interleukin-1 receptor type 2; IL-1R-beta; antigen CDw121b; IL-1 type II receptor; interleukin-1 receptor beta; interleukin-1 receptor type II; CD121 antigen-like family member B; type II interleukin-1 receptor, beta; interleukin 1 receptor type II varian
• UniProt Protein Name: Interleukin-1 receptor type 2
• Protein Family: Interleukin-1 receptor
• UniProt Gene Name: IL1R2
• UniProt Synonym Gene Names: IL1RB; IL-1R-2; IL-1RT-2; IL-1RT2; IL-1R-beta; mIL-1R2; mIL-1RII; sIL-1R2; sIL-1RII
• UniProt Entry Name: IL1R2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage. [provided by RefSeq, May 2012]

Uniprot Description

IL-1RB: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; interleukin-1, Type II, blocking receptor activity; interleukin-1 receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; immune response