Western Blot (WB)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human HMGB2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human High Mobility Group Box Protein 2 Polyclonal Antibody | anti-HMGB2 antibody
Polyclonal Antibody to High Mobility Group Box Protein 2 (HMGB2)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS AFFLFCSEHR PKIKSEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEP
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human HMGB2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human HMGB2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded liver tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq, Jul 2008]
Uniprot Description
HMGB2: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Belongs to the HMGB family.
Protein type: DNA-binding; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 4q34.1
Cellular Component: cell; condensed chromosome; cytoplasm; extracellular space; nuclear chromatin; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm; protein complex
Molecular Function: chemoattractant activity; damaged DNA binding; DNA binding; DNA binding transcription factor activity; DNA binding, bending; double-stranded DNA binding; drug binding; four-way junction DNA binding; non-sequence-specific DNA binding, bending; protein binding; protein domain specific binding; RAGE receptor binding; RNA binding; single-stranded DNA binding; transcription factor binding; transcription regulatory region DNA binding
Biological Process: apoptotic DNA fragmentation; chromatin organization; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; DNA geometric change; DNA ligation involved in DNA repair; DNA topological change; inflammatory response to antigenic stimulus; innate immune response; male gonad development; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of transcription, DNA-dependent; nucleosome assembly; positive chemotaxis; positive regulation of DNA binding; positive regulation of endothelial cell proliferation; positive regulation of erythrocyte differentiation; positive regulation of innate immune response; positive regulation of interferon-beta production; positive regulation of megakaryocyte differentiation; positive regulation of nuclease activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of neurogenesis; regulation of stem cell proliferation; regulation of transcription from RNA polymerase II promoter; response to drug; response to lipopolysaccharide; response to steroid hormone; spermatid nuclear differentiation; transcription, DNA-dependent; V(D)J recombination
Research Articles on HMGB2
Similar Products
Product Notes
The HMGB2 hmgb2 (Catalog #AAA2026089) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to High Mobility Group Box Protein 2 (HMGB2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's High Mobility Group Box Protein 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the HMGB2 hmgb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS AFFLFCSEHR PKIKSEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEP. It is sometimes possible for the material contained within the vial of "High Mobility Group Box Protein 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
