RAD51D recombinant protein

RAD51D,1-328aa, Human, His tag, E Coli

Cat. Num. AAA205614

• Gene Names: RAD51D; TRAD; R51H3; BROVCA4; RAD51L3
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: RAD51D; 1-328aa; Human; His tag; E Coli; RAD51 homolog D isoform 1; BROVCA4; R51H3; RAD51L3; TRAD; RAD51D recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGVLRVG LCPGLTEEMI QLLRSHRIKT VVDLVSADLE EVAQKCGLSY KALVALRRVL LAQFSAFPVN GADLYEELKT STAILSTGIG SLDKLLDAGL YTGEVTEIVG GPGSGKTQVC LCMAANVAHG LQQNVLYVDS NGGLTASRLL QLLQAKTQDE EEQAEALRRI QVVHAFDIFQ MLDVLQELRG TVAQQVTGSS GTVKVVVVDS VTAVVSPLLG GQQREGLALM MQLARELKTL ARDLGMAVVV TNHITRDRDS GRLKPALGRS WSFVPSTRIL LDTIEGAGAS GGRRMACLAK SSRQPTGFQE MVDIGTWGTS EQSATLQGDQ T
• Sequence Length: 328

• Applicable Applications for RAD51D recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RAD51D recombinant protein

RAD51D is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Recombinant human RAD51D protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RAD51D recombinant protein

Epigenomics (Transcription & Translation)

References

Masson J.Y., et al. (2001) Genes Dev. 15:3296-3307; Liu N., et al. (2002) Nucleic Acids Res. 30:1009-1015;

NCBI and Uniprot Product Information

• NCBI GI #: 217416415
• NCBI GeneID: 5892
• NCBI Accession #: NP_002869
• NCBI GenBank Nucleotide #: NM_002878.3
• UniProt Accession #: O75771; O43537; O60355; O75196; O75847; O75848; O76073; O76085; O94908; Q9UFU5; B4DJU7; E1P637
• Molecular Weight: 37.4 kDa (351aa) confirmed by MALDI-TOF
• NCBI Official Full Name: DNA repair protein RAD51 homolog 4 isoform 1
• NCBI Official Synonym Full Names: RAD51 paralog D
• NCBI Official Symbol: RAD51D
• NCBI Official Synonym Symbols: TRAD; R51H3; BROVCA4; RAD51L3
• NCBI Protein Information: DNA repair protein RAD51 homolog 4
• UniProt Protein Name: DNA repair protein RAD51 homolog 4
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD51D
• UniProt Synonym Gene Names: RAD51L3

NCBI Description

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.