Rabbit PDE8A Polyclonal Antibody | anti-PDE8A antibody

Rabbit Polyclonal to Human PDE8A

Cat. Num. AAA240110

• Gene Names: PDE8A; HsT19550
• Reactivity: Gibbon, Dog, Gorilla, Horse, Human, Pig, Rabbit; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: PDE8A; Polyclonal Antibody; Rabbit Polyclonal to Human PDE8A; Anti-PDE8A Antibody (N-Terminus) IHC-plus; Phosphodiesterase 8A; HsT19550; Human PDE8A; anti-PDE8A antibody

• Host: Rabbit
• Reactivity: Gibbon, Dog, Gorilla, Horse, Human, Pig, Rabbit; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human PDE8A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 757

• Applicable Applications for anti-PDE8A antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (10 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic 16 amino acid peptide from near N-terminus of human PDE8A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Panda, Horse, Rabbit, Pig (100%); Marmoset (94%).
• Immunogen Type: Synthetic peptide
• Immunogen: PDE8A antibody was raised against synthetic 16 amino acid peptide from near N-terminus of human PDE8A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Panda, Horse, Rabbit, Pig (100%); Marmoset (94%); Mouse, Rat, Bovine, Elephant, Opossum (88%); Platypus, Zebrafish (81%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-PDE8A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-PDE8A antibody

Phosphodiesterase 8A, a high affinity, cAMP-specific phosphodiesterase, has been implicated in penile erectile dysfunction. PDE8A activity is inhibited by dipyridamole, but not by rolipram, zaprinast, or IBMX. At least five splice variants and four protein isoforms have been reported.

Product Categories/Family for anti-PDE8A antibody

Family: Phosphodiesterase; Subfamily: PDE - cAMP-specific; high affinity

NCBI and Uniprot Product Information

• NCBI GI #: 341823698
• NCBI GeneID: 5151
• NCBI Accession #: NP_001230066.1
• NCBI GenBank Nucleotide #: NM_001243137.1
• UniProt Accession #: O60658; Q6P9H3; Q969I1; Q96PC9; Q96PD0; Q96PD1; Q96T71; Q9UMB7; Q9UMC3; B3KXE6; H0YMZ7
• Molecular Weight: 86,049 Da
• NCBI Official Full Name: high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform 3
• NCBI Official Synonym Full Names: phosphodiesterase 8A
• NCBI Official Symbol: PDE8A
• NCBI Official Synonym Symbols: HsT19550
• NCBI Protein Information: high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A; cAMP-specific cyclic nucleotide phosphodiesterase 8A
• UniProt Protein Name: High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
• Protein Family: High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE8A
• UniProt Entry Name: PDE8A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

PDE8A: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Phosphodiesterase; EC 3.1.4.53

Chromosomal Location of Human Ortholog: 15q25.3

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; signal transducer activity; metal ion binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: cyclic nucleotide metabolic process; regulation of transcription, DNA-dependent; cAMP catabolic process; two-component signal transduction system (phosphorelay)