DNA repair protein RAD51 homolog 4 (RAD51D) Recombinant Protein | RAD51D recombinant protein

Recombinant Human DNA repair protein RAD51 homolog 4 (RAD51D)

Cat. Num. AAA1172615

• Gene Names: RAD51D; TRAD; R51H3; BROVCA4; RAD51L3
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA repair protein RAD51 homolog 4 (RAD51D); Recombinant Human DNA repair protein RAD51 homolog 4 (RAD51D); DNA repair protein RAD51 homolog 4; R51H3; RAD51 homolog D; RAD51-like protein 3; TRAD; RAD51D recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-328aa; Full Length
• Sequence: MGVLRVGLCPGLTEEMIQLLRSHRIKTVVDLVSADLEEVAQKCGLSYKALVALRRVLLAQFSAFPVNGADLYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCMAANVAHGLQQNVLYVDSNGGLTASRLLQLLQAKTQDEEEQAEALRRIQVVHAFDIFQMLDVLQELRGTVAQQVTGSSGTVKVVVVDSVTAVVSPLLGGQQREGLALMMQLARELKTLARDLGMAVVVTNHITRDRDSGRLKPALGRSWSFVPSTRILLDTIEGAGASGGRRMACLAKSSRQPTGFQEMVDIGTWGTSEQSATLQGDQT
• Sequence Length: 328

• Species: Homo sapiens (Human)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

SDS-PAGE

Related Product Information for RAD51D recombinant protein

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.

Product Categories/Family for RAD51D recombinant protein

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 217272816
• NCBI GeneID: 5892
• NCBI Accession #: NP_001136043.1
• NCBI GenBank Nucleotide #: NM_001142571.1
• UniProt Accession #: O75771; O43537; O60355; O75196; O75847; O75848; O76073; O76085; O94908; Q9UFU5; B4DJU7; E1P637
• Molecular Weight: 62 kDa
• NCBI Official Full Name: DNA repair protein RAD51 homolog 4 isoform 6
• NCBI Official Synonym Full Names: RAD51 paralog D
• NCBI Official Symbol: RAD51D
• NCBI Official Synonym Symbols: TRAD; R51H3; BROVCA4; RAD51L3
• NCBI Protein Information: DNA repair protein RAD51 homolog 4; RAD51 homolog D; RAD51-like protein 3; recombination repair protein
• UniProt Protein Name: DNA repair protein RAD51 homolog 4
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD51D
• UniProt Synonym Gene Names: RAD51L3
• UniProt Entry Name: RA51D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene. [provided by RefSeq, Jan 2011]

Uniprot Description

RAD51L3: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Defects in RAD51D are a cause of susceptibility to familial breast-ovarian cancer type 4 (BROVCA4). A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Belongs to the RecA family. RAD51 subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: centrosome; chromosome, telomeric region; cytoplasm; replication fork; nucleus

Molecular Function: DNA-dependent ATPase activity; gamma-tubulin binding; protein binding; DNA binding; four-way junction DNA binding; ATP binding; single-stranded DNA binding

Biological Process: meiotic recombination; strand invasion; DNA repair; telomere maintenance; double-strand break repair via homologous recombination

Disease: Breast-ovarian Cancer, Familial, Susceptibility To, 4