Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Lt. Chain Rabbit Recombinant Monoclonal Antibody (IGKC /1999R).)
Rabbit anti-Human Kappa Light Chain (B-Cell Marker) Antibody | anti-IGKC antibody
Kappa Light Chain (B-Cell Marker) Recombinant Rabbit Monoclonal Antibody [Clone IGKC/1999R]
Reactivity
Human
Synonyms
Kappa Light Chain (B-Cell Marker); Kappa Light Chain (B-Cell Marker) Recombinant Rabbit Monoclonal Antibody [Clone IGKC/1999R]; HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM; anti-IGKC antibody
Host
Rabbit
Reactivity
Human
Isotype
IgG
Specificity
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Form/Format
200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Application Notes
Western Blotting (0.5-1.0ug/ml); Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 min at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunogen
Recombinant full-length human Ig kappa chain
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen
Cellular Localization
Cell Surface, cytoplasmic and secreted
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Lt. Chain Rabbit Recombinant Monoclonal Antibody (IGKC /1999R).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Lt. Chain Rabbit Recombinant Monoclonal Antibody (IGKC /1999R).)
SDS-Page
(SDS-PAGE Analysis of Purified Kappa Lt. Chain Rabbit Recombinant Monoclonal Antibody (IGKC /1999R).)
SDS-Page
(SDS-PAGE Analysis of Purified Kappa Lt. Chain Rabbit Recombinant Monoclonal Antibody (IGKC /1999R).)
References
1.Takahashi H et. al. Pathol Res Prac 189:300-311 (1993).2.Momose H et. al. Hum Pathol. 23:1115-1119 (1992).
NCBI and Uniprot Product Information
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Product Notes
The IGKC (Catalog #AAA439995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kappa Light Chain (B-Cell Marker) Recombinant Rabbit Monoclonal Antibody [Clone IGKC/1999R] reacts with Human and may cross-react with other species as described in the data sheet. Western Blotting (0.5-1.0ug/ml); Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 min at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the IGKC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kappa Light Chain (B-Cell Marker), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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