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Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Human Tonsil stained with Kappa Light Chain Rabbit Recombinant Monoclonal (KLC2289R).)

Rabbit Kappa LightChain/IGKC Monoclonal Antibody | anti-IGKC antibody

Kappa LightChain/IGKC (B-Cell Marker)

Reactivity
Human. Others not known.
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
Kappa LightChain/IGKC; Monoclonal Antibody; Kappa LightChain/IGKC (B-Cell Marker); HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM; anti-IGKC antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG
Clone Number
KLC2289R
Specificity
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
117
Applicable Applications for anti-IGKC antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin
Application Notes
FC/FACS: 0.5-1ug/million cells
IHC-F: 0.5-1.0ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Recombinant full-length human Ig kappa chain
Cellular Localization
Cell Surface, Cytoplasmic and secreted
Hu-chromosome Location
2p11.2
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded Human Tonsil stained with Kappa Light Chain Rabbit Recombinant Monoclonal (KLC2289R).)

Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Human Tonsil stained with Kappa Light Chain Rabbit Recombinant Monoclonal (KLC2289R).)

SDS-Page

(SDS-PAGE Analysis of Purified Kappa Light Chain Rabbit Recombinant Monoclonal (KLC2289R).)

SDS-Page (SDS-PAGE Analysis of Purified Kappa Light Chain Rabbit Recombinant Monoclonal (KLC2289R).)
References
Nakamura T et. al. Proc Natl AcadSci U S A. 1992; 89(18):8522-6. KiyotakiM et. al. J Immunol. 1987; 138(12):4150-8.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~22.5kDa
NCBI Official Full Name
Immunoglobulin kappa variable 1D-16
UniProt Protein Name
Immunoglobulin kappa variable 1D-16
UniProt Gene Name
IGKV1D-16

Uniprot Description

V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:20176268, PubMed:17576170).

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Product Notes

The IGKC igkv1d-16 (Catalog #AAA4380042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kappa LightChain/IGKC (B-Cell Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Kappa LightChain/IGKC can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin. FC/FACS: 0.5-1ug/million cells IHC-F: 0.5-1.0ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the IGKC igkv1d-16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kappa LightChain/IGKC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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