Mouse Kappa Light Chain Monoclonal Antibody | anti-IGKC antibody

Kappa Light Chain (B-Cell Marker)

Cat. Num. AAA4380038

• Reactivity: Human. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: Kappa Light Chain; Monoclonal Antibody; Kappa Light Chain (B-Cell Marker); HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM; anti-IGKC antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: rKLC264
• Specificity: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 117

• Applicable Applications for anti-IGKC antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 0.5-1ug/ml; WB: 0.5-1.0ug/ml; IHC-F: 0.5-1.0ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant full-length human Ig kappa chain
• Cellular Localization: Cell Surface, Cytoplasmic and secreted
• Hu-chromosome Location: 2p11.2
• Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Mouse Recombinant Monoclonal Antibody (rKLC264).)

SDS-Page

(SDS-PAGE Analysis of Purified Kappa Light Chain Mouse Recombinant Monoclonal Antibody (rKLC264).)

Testing Data

(Analysis of Protein Array containing >19, 000 full-length human proteins using Kappa Light Chain Mouse Monoclonal Antibody (rKLC/264) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

References

1.Takahashi H et. al. Pathol Res Prac 189:300-311 (1993).2.Momose H et. al. Hum Pathol. 23:1115-1119 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 857434459
• NCBI GeneID: 449609; 3514
• UniProt Accession #: P01601; A0A0B4J1Z3
• Molecular Weight: ~22.5kDa
• NCBI Official Full Name: Immunoglobulin kappa variable 1D-16
• UniProt Protein Name: Immunoglobulin kappa variable 1D-16
• UniProt Gene Name: IGKV1D-16

Uniprot Description

V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:20176268, PubMed:17576170).