Mouse anti-Human Lambda Light Chain Monoclonal Antibody | anti-IGL antibody

Lambda Light Chain (B-Cell Marker)

Cat. Num. AAA4381012

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab conjugated to CF488
• Synonyms: Lambda Light Chain; Monoclonal Antibody; Lambda Light Chain (B-Cell Marker); Bence Jones Protein; BJP; IGLC 1/2/3; Mcg Marker; Paraprotein; anti-IGL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: HP6054
• Specificity: This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.
• Sequence Length: 111

• Applicable Applications for anti-IGL antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 5ul per test per one million cells or 5ul per 100ul of whole blood; IF: 1:50-1:100
• Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
• Immunogen: Purified human IgG myeloma proteins coupled to polyaminostyren microbeads
• Cellular Localization: Cell Surface, Cytoplasmic and Secreted
• Hu-chromosome Location: 22q11.22
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunofluorescence (IF)

(Confocal Immunofluorescent analysis of Ramos cells using CF488-labeled Lambda Light Chain Monoclonal Antibody (HP6054) (Green). DAPI was used to stain the cell nuclei (blue).)

Immunofluorescence (IF)

(Confocal Immunofluorescent analysis of Ramos cells using CF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control))

References

Reimer et al. Hybridoma 3: 263, 1984.

NCBI and Uniprot Product Information

• NCBI GI #: 126541
• UniProt Accession #: P01701; P01842
• Molecular Weight: ~22.5kDa
• NCBI Official Full Name: Ig lambda chain V-I region NEW
• UniProt Protein Name: Ig lambda chain V-I region NEW
• UniProt Entry Name: LV103_HUMAN

Uniprot Description

IGLV1-51: V segment of the variable region of immunoglobulins light chain that participates to the antigen recognition. Immunoglobulins (Igs), also known as antibodies, are membrane- bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors which, upon binding of a specific antigen (Ag), trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable region of one heavy chain, together with that of its associated light chain. Thus, each Ig has two antigen binding sites with remarkable affinity for a particular antigen. The variable regions are assembled by a process called V(D)J recombination and can then be subjected to somatic hypermutation after exposure to antigen to allow affinity maturation for a particular Ag (PubMed:20176268, PubMed:17576170). {ECO:0000303|PubMed:17576170, ECO:0000303|PubMed:20176268, ECO:0000303|PubMed:22158414}. Immunoglobulins are composed of two identical heavy chains and two identical light chains, linked by disulfide bonds. {ECO:0000303|PubMed:20176268}