NuDT16 recombinant protein

NuDT16, 1-195aa, Human, His tag, E Coli

Cat. Num. AAA203867

• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: NuDT16; 1-195aa; Human; His tag; E Coli; Nudix type motif 16; NuDT16 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100mM NaCl
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAGARRLELG EALALGSGWR HACHALLYAP DPGMLFGRIP LRYAILMQMR FDGRLGFPGG FVDTQDRSLE DGLNRELREE LGEAAAAFRV ERTDYRSSHV GSGPRVVAHF YAKRLTLEEL LAVEAGATRA KDHGLEVLGL VRVPLYTLRD GVGGLPTFLE NSFIGSAREQ LLEALQDLGL LQSGSISGLK IPAHH
• Sequence Length: 195

• Applicable Applications for NuDT16 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Domain: 1-195aa
• Preparation and Storage: Can be stored at 4°C short term (1-2 weeks).; For long term storage, aliquot and store at -20°C or -80°C.; Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for NuDT16 recombinant protein

NuDT16 is nuclear nucleoside diphosphatase proteins localized within foci in the nucleolus and nucleoplasm. This is a RNA-decapping enzyme that binds specifically to u8 snoRNA. It can remove m7G and m227G caps from RNAs, rendering them substrates for 5'-3' exonucleases for degradation in vivo. Recombinant human NuDT16 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for NuDT16 recombinant protein

Enzymes & Proteases

References

Peculis B.A., et al. (2007) J. Biol. Chem. 282:24792-24805; Ghosh T., et al. (2004) Mol. Cell 13:817-828;

NCBI and Uniprot Product Information

• NCBI GI #: 285026434
• NCBI GeneID: 131870
• NCBI Accession #: NP_689608
• NCBI GenBank Nucleotide #: NM_152395.2
• UniProt Accession #: Q96DE0; Q96N82; B4E3B4; E9PED4; F5GYJ1
• Molecular Weight: 23.4kDa (215aa), confirmed by MALDI-TOF
• NCBI Official Full Name: U8 snoRNA-decapping enzyme isoform 2
• NCBI Official Synonym Full Names: nudix hydrolase 16
• NCBI Official Symbol: NUDT16
• NCBI Protein Information: U8 snoRNA-decapping enzyme
• UniProt Protein Name: U8 snoRNA-decapping enzyme
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT16
• UniProt Synonym Gene Names: IDPase; Nudix motif 16

Uniprot Description

RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms (PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP (PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions (PubMed:20385596).