Rabbit Isocitrate dehydrogenase [NAD] subunit beta Polyclonal Antibody | anti-IDH3B antibody

Rabbit anti-human Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial polyclonal Antibody

Cat. Num. AAA1488323

• Gene Names: IDH3B; RP46; H-IDHB
• Reactivity: Human, Mouse. Other species are not tested. Please decide the specificity by homology.
• Applications: ELISA, Western Blot
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: Isocitrate dehydrogenase [NAD] subunit beta; Polyclonal Antibody; Rabbit anti-human Isocitrate dehydrogenase [NAD] subunit beta; mitochondrial polyclonal Antibody; Isocitric dehydrogenase subunit beta NAD(+)-specific ICDH subunit beta IDH3B; anti-IDH3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse. Other species are not tested. Please decide the specificity by homology.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 385

• Applicable Applications for anti-IDH3B antibody: ELISA (EIA), Western Blot (WB)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial protein
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial antibody at 2ug/mllane 1:Hela whole cell lysatelane 2:mouse kidney tissuelane 3:mouse gonad tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 43,42,26 kDaObserved band size: 42 kDa)

NCBI and Uniprot Product Information

• NCBI GI #: 28178821
• NCBI GeneID: 3420
• NCBI Accession #: NP_008830.2
• NCBI GenBank Nucleotide #: NM_006899.3
• UniProt Accession #: O43837; O95106; Q5JXS8; Q9NQ06; Q9NQ07; Q9NUZ0; Q9UEX0; Q9UG99; B2RDR1; D3DVX2; D3DVX3
• Molecular Weight: 25,555 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 3 (NAD+) beta
• NCBI Official Symbol: IDH3B
• NCBI Official Synonym Symbols: RP46; H-IDHB
• NCBI Protein Information: isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH3B
• UniProt Entry Name: IDH3B_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IDH3B: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 has apparent tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 1.1.1.41; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: mitochondrial matrix; mitochondrion; nucleus

Molecular Function: electron carrier activity; isocitrate dehydrogenase (NAD+) activity; magnesium ion binding; NAD binding

Biological Process: 2-oxoglutarate metabolic process; cellular metabolic process; isocitrate metabolic process; NADH metabolic process; tricarboxylic acid cycle

Disease: Retinitis Pigmentosa 46