Human V-type proton ATPase catalytic subunit A ELISA Kit | ATP6V1A elisa kit

Human V-type proton ATPase catalytic subunit A ELISA Kit

Cat. Num. AAA2881479

• Gene Names: ATP6V1A; HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
• Reactivity: Human
• Synonyms: V-type proton ATPase catalytic subunit A; Human V-type proton ATPase catalytic subunit A ELISA Kit; V-ATPase subunit A; V-ATPase 69 kDa subunit; Vacuolar ATPase isoform VA68; Vacuolar proton pump subunit alpha; ATP6V1A; ATP6A1; ATP6V1A elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human V-type proton ATPase catalytic subunit A
• Sequence Length: 617

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.7%
• Inter-assay Precision: Inter-Assay CV: <=9.6%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP6V1A elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19913424
• NCBI GeneID: 523
• NCBI Accession #: NP_001681.2
• NCBI GenBank Nucleotide #: NM_001690.3
• UniProt Accession #: P38606; Q53YD9; Q96DY6; Q9UHY3; B2RBR8; B7Z1R5; D3DN75
• Molecular Weight: 64,736 Da
• NCBI Official Full Name: V-type proton ATPase catalytic subunit A
• NCBI Official Synonym Full Names: ATPase H+ transporting V1 subunit A
• NCBI Official Symbol: ATP6V1A
• NCBI Official Synonym Symbols: HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
• NCBI Protein Information: V-type proton ATPase catalytic subunit A
• UniProt Protein Name: V-type proton ATPase catalytic subunit A
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V1A
• UniProt Synonym Gene Names: ATP6A1; ATP6V1A1; VPP2; V-ATPase subunit A
• UniProt Entry Name: VATA_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1A: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the ATPase alpha/beta chains family.

Protein type: EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: apical plasma membrane; cytosol; integral to plasma membrane; lysosomal membrane; microvillus; mitochondrion; myelin sheath; plasma membrane; proton-transporting two-sector ATPase complex

Molecular Function: ATP binding; hydrogen ion transporting ATPase activity, rotational mechanism

Biological Process: ATP hydrolysis coupled proton transport; ATP metabolic process; cell redox homeostasis; cellular iron ion homeostasis; insulin receptor signaling pathway; transferrin transport; transmembrane transport; transport