Goat ACTA2 / Smooth Muscle Actin Polyclonal Antibody | anti-ACTA2 antibody

Goat Polyclonal to Human ACTA2 / Smooth Muscle Actin

Cat. Num. AAA244295

• Gene Names: ACTA2; AAT6; ACTSA; MYMY5
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ACTA2 / Smooth Muscle Actin; Polyclonal Antibody; Goat Polyclonal to Human ACTA2 / Smooth Muscle Actin; ACTA2 | ACTVS | Alpha-actin-2 | Alpha-cardiac actin | AAT6 | Actin; aortic smooth muscle | ACTSA | Smooth Muscle Actin | MYMY5 | SMA; anti-ACTA2 antibody

• Host: Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
• Clonality: Polyclonal
• Specificity: Human ACTA2. Variants NP_001604.1 and NP_001135417.1 encode the same protein.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 377

• Applicable Applications for anti-ACTA2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC - Paraffin: (2.5 µg/ml); WB: (0.1 - 1 µg/ml); Peptide Enzyme-Linked Immunosorbent Assay (1:64000); ; (Optimal dilutions to be determined by the researcher)
• Target: Human ACTA2 / Smooth Muscle Actin
• Antigen Type: Synthetic Peptide
• Immunogen Species: ACTA2/ Smooth Muscle Actin antibody was raised against Human
• Immunogen: A synthetic peptide corresponding to the N-Terminus of human Alpha-Smooth Muscle Actin (ACTA2).
• Epitope: aa1-50
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ACTA2 antibody IHC of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ACTA2 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Western Blot (WB)

(Antibody staining (0.1 ug/ml) of Human Duodenum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Related Product Information for anti-ACTA2 antibody

Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

NCBI and Uniprot Product Information

• NCBI GI #: 4501883
• NCBI GeneID: 59
• NCBI Accession #: NP_001604.1
• NCBI GenBank Nucleotide #: NM_001613.2
• UniProt Accession #: P62736; P03996; P04108; Q6FI19; B2R8A4
• NCBI Official Full Name: actin, aortic smooth muscle
• NCBI Official Synonym Full Names: actin, alpha 2, smooth muscle, aorta
• NCBI Official Symbol: ACTA2
• NCBI Official Synonym Symbols: AAT6; ACTSA; MYMY5
• NCBI Protein Information: actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
• UniProt Protein Name: Actin, aortic smooth muscle
• Protein Family: Actin
• UniProt Gene Name: ACTA2
• UniProt Synonym Gene Names: ACTSA; ACTVS
• UniProt Entry Name: ACTA_HUMAN