Testing Data
Rabbit anti-Human ER (pS167) Antibody | anti-ER (pS167) antibody
Rabbit anti ER (pSer 167), Phosphospecific
Reactivity
Human
Applications
ELISA, Western Blot
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Synonyms
ER (pS167); Rabbit anti ER (pSer 167); Phosphospecific; Rabbit anti-ER (pSer167) (Phosphospecific); Estrogen Receptor; anti-ER (pS167) antibody
Host
Rabbit
Reactivity
Human
Specificity
This antibody recognizes ~67 kDa of human estrogen receptor at the phosphorylation site of Ser 167. It does not cross react to non-hosphorylated peptide. This antibody reacts with human. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-ER (pS167) antibody
ELISA, Western Blot
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:200
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:200
Antigen Preparation
A synthetic peptide surrounding the epitope -LASTN- with a phosphorylation site of Ser167 of human estrogen receptor.
Positive Control
MCF-7
Cellular Location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-ER (pS167) antibody
ER (estrogen receptor) belongs to a member of the steroid receptor superfamily. It contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by its phosphorylation at multiple sites, such as serine 104, 106, 118, 167 and 305. Phosphorylation of certain serine at ER may relate to breast cancer process and on-going treatment.
References
Dutertre M, Smith CL. 2003. Ligand-independent interactions of p160/steroid receptor coactivators and CREB-binding protein (CBP) with estrogen receptor-alpha: regulation by phosphorylation sites in the A/B region depends on other receptor domains. Mol Endocrinol 17:1296-314.
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Product Notes
The ER (pS167) (Catalog #AAA462045) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti ER (pSer 167), Phosphospecific reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ER (pS167) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:200. Researchers should empirically determine the suitability of the ER (pS167) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ER (pS167), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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