Rat Adenosine A3 Receptor (ADORA3) ELISA Kit | ADORA3 elisa kit

Rat Adenosine A3 Receptor (ADORA3) ELISA Kit

Cat. Num. AAA2025108

• Gene Names: ADORA3; A3AR
• Reactivity: Rat
• Synonyms: Adenosine A3 Receptor (ADORA3); Rat Adenosine A3 Receptor (ADORA3) ELISA Kit; A3AR; AD026; ADORA3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenosine A3 Receptor (ADORA3).; No significant cross-reactivity or interference between Adenosine A3 Receptor (ADORA3) and analogues was observed.
• Sequence Length: 8

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine A3 Receptor (ADORA3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine A3 Receptor (ADORA3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADORA3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine A3 Receptor (ADORA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine A3 Receptor (ADORA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine A3 Receptor (ADORA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosine A3 Receptor (ADORA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ADORA3 elisa kit

Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 4262078
• NCBI GeneID: 140
• Molecular Weight: 847 Da
• NCBI Official Full Name: adenosine A3 receptor, partial
• NCBI Official Synonym Full Names: adenosine A3 receptor
• NCBI Official Symbol: ADORA3
• NCBI Official Synonym Symbols: A3AR
• NCBI Protein Information: adenosine receptor A3
• UniProt Protein Name: Adenosine A3 receptor protein
• Protein Family: Adenosine receptor
• UniProt Gene Name: adenosine A3 receptor
• UniProt Entry Name: Q6LD47_HUMAN

NCBI Description

This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Alternative splicing results in multiple transcript variants. This gene shares its 5' terminal exon with some transcripts from overlapping GeneID:57413, which encodes an immunoglobulin domain-containing protein. [provided by RefSeq, Nov 2014]

Uniprot Description

ADORA3: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: mast cell granule; integral to plasma membrane; plasma membrane

Molecular Function: adenosine receptor activity, G-protein coupled

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; histamine secretion by mast cell; positive regulation of leukocyte migration; adenylate cyclase activation; response to wounding; positive regulation of calcium-mediated signaling; positive regulation of mast cell degranulation; signal transduction; inflammatory response; adenosine receptor signaling pathway; regulation of heart contraction; positive regulation of inflammatory response