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Western Blot (WB) (INA Antibody western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the INA antibody detected the INA protein (arrow).)

Mouse INA Monoclonal Antibody | anti-INA antibody

INA Antibody

Gene Names
INA; NEF5; NF-66; TXBP-1
Reactivity
Mouse (Predicted Reactivity: Bovine, Rat)
Applications
Western Blot, ELISA, Immunofluorescence, Immunohistochemistry
Synonyms
INA; Monoclonal Antibody; INA Antibody; Alpha-internexin; Alpha-Inx; 66 kDa neurofilament protein; NF-66; Neurofilament-66; Neurofilament 5; NEF5; anti-INA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Mouse (Predicted Reactivity: Bovine, Rat)
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
257CT7.1.2
Specificity
This INA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 290-319 amino acids from human INA.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
290-319
Sequence Length
499
Applicable Applications for anti-INA antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB~~1:100~250
IF~~1:10~50
IHC~~1:10~50
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(INA Antibody western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the INA antibody detected the INA protein (arrow).)

Western Blot (WB) (INA Antibody western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the INA antibody detected the INA protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of INA Antibody with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of INA Antibody with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(INA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of INA Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (INA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of INA Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-INA antibody
Neurofilaments are type IV intermediate filament
heteropolymers composed of light, medium, and heavy chains.
Neurofilaments comprise the axoskeleton and they functionally
maintain the neuronal caliber. They may also play a role in
intracellular transport to axons and dendrites. This gene is a
member of the intermediate filament family and is involved in the
morphogenesis of neurons.
Product Categories/Family for anti-INA antibody
References
Leermakers, F.A., et al. Eur. Biophys. J. 39(9):1323-1334(2010)
Martins-de-Souza, D., et al. J Psychiatr Res 43(11):978-986(2009)
Ducray, F., et al. Neurology 72(2):156-161(2009)
Willoughby, V., et al. Appl. Immunohistochem. Mol. Morphol. 16(4):344-348(2008)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55391
NCBI Official Full Name
alpha-internexin
NCBI Official Synonym Full Names
internexin neuronal intermediate filament protein, alpha
NCBI Official Symbol
INA
NCBI Official Synonym Symbols
NEF5; NF-66; TXBP-1
NCBI Protein Information
alpha-internexin
UniProt Protein Name
Alpha-internexin
Protein Family
UniProt Gene Name
INA
UniProt Synonym Gene Names
NEF5; Alpha-Inx; NF-66; Neurofilament-66
UniProt Entry Name
AINX_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]

Uniprot Description

INA: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; extracellular space; intermediate filament cytoskeleton; nuclear membrane; neurofilament

Molecular Function: structural constituent of cytoskeleton

Biological Process: substantia nigra development; neurofilament cytoskeleton organization and biogenesis; cell differentiation

Research Articles on INA

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Product Notes

The INA ina (Catalog #AAA9200106) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 290-319. The INA Antibody reacts with Mouse (Predicted Reactivity: Bovine, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's INA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC). WB~~1:100~250 IF~~1:10~50 IHC~~1:10~50. Researchers should empirically determine the suitability of the INA ina for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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