Rabbit Pulmonary surfatcant-associated protein A, SP-A ELISA Kit | SP-A elisa kit

Rabbit Pulmonary surfatcant-associated protein A, SP-A ELISA Kit

Cat. Num. AAA730836

• Gene Names: Sftpa1; SP-A; Sftp1; Sftp-1
• Reactivity: Rabbit
• Synonyms: Pulmonary surfatcant-associated protein A; SP-A; Rabbit Pulmonary surfatcant-associated protein A; SP-A ELISA Kit; Rabbit Pulmonary surfatcant-associated protein A (SP-A) ELISA Kit; SP-A ELISA Kit (Rabbit); SP-A elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This SPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit SPA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SP-A elisa kit

Principle of the assay: SPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SPA antibody and an SPA-HRP conjugate. The assay sample and buffer are incubated together with SPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPA concentration since SPA from samples and SPA-HRP conjugate compete for the anti-SPA antibody binding site. Since the number of sites is limited, as more sites are occupied by SPA from the sample, fewer sites are left to bind SPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SP-A elisa kit

Rabbit ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 260453
• NCBI GeneID: 20387
• UniProt Accession #: P35242; Q6P8S2; Q9CQI1
• Molecular Weight: 26,157 Da
• NCBI Official Full Name: SP-A
• NCBI Official Synonym Full Names: surfactant associated protein A1
• NCBI Official Symbol: Sftpa1
• NCBI Official Synonym Symbols: SP-A; Sftp1; Sftp-1
• NCBI Protein Information: pulmonary surfactant-associated protein A; PSAP; PSP-A; surfactant pulmonary associated protein A1
• UniProt Protein Name: Pulmonary surfactant-associated protein A
• UniProt Gene Name: Sftpa1
• UniProt Synonym Gene Names: Sftp-1; Sftp1; Sftpa
• UniProt Entry Name: SFTPA_MOUSE

Uniprot Description

SFTPA2: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Defects in SFTPA2 are a cause of pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. Belongs to the SFTPA family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: multivesicular body; extracellular space; rough endoplasmic reticulum; cytoplasmic vesicle

Biological Process: positive regulation of phagocytosis