Testing Data
Rabbit SHP1 (Ser-591) Polyclonal Antibody
SHP1 (Ser-591), phospho-specific
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot
Purity
Affinity Purified
Synonyms
SHP1 (Ser-591); Polyclonal Antibody; phospho-specific; PTP-1C; Protein-tyrosine phosphatase 1C; SH-PTP1; SHP-1; anti-SHP1 (Ser-591) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to a non-specific phospho-serine peptide then affinity-purified using phospho-SHP1 (Ser-591) peptide. The antibody detects a 68 kDa* band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as wellas A431 cells treated with EGF.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-SHP1 (Ser-591) antibody
ELISA, Western Blot
Application Notes
ELISA: 1:2000
Western Blot: 1:1000
Western Blot: 1:1000
Immunogen
Phospho-SHP1 (Ser-591) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 591 in human SHP1. The
sequence is highly conserved in rat and mouse SHP1.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for anti-SHP1 (Ser-591) antibody
SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKC-alpha and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.
References
• Jones, M.L. et al. (2004) J. Biol. Chem. 279(39):40475.
• Zhang, Z. et al. (2003) J. Biol. Chem. 278(7):4668.
• Zhang, J. et al. (2000) Semin. Immunol. 12:361.
• Simoneau, M. et al. (2008) J Biol Chem Epub:July 10. (WB: S591A transfectants)
• Zhang, Z. et al. (2003) J. Biol. Chem. 278(7):4668.
• Zhang, J. et al. (2000) Semin. Immunol. 12:361.
• Simoneau, M. et al. (2008) J Biol Chem Epub:July 10. (WB: S591A transfectants)
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Product Notes
The SHP1 (Ser-591) (Catalog #AAA472061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHP1 (Ser-591), phospho-specific reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SHP1 (Ser-591) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. ELISA: 1:2000 Western Blot: 1:1000. Researchers should empirically determine the suitability of the SHP1 (Ser-591) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHP1 (Ser-591), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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