Neuroserpin Recombinant Protein | SerpinI1 recombinant protein

Neuroserpin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123034

• Gene Names: Serpini1; Ns; PI12; PI-12; Spi17; AI837402
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Neuroserpin; Neuroserpin Protein; Mouse; Recombinant (His Tag); Mouse SerpinI1/Neuroserpin Protein (His Tag); AI837402 Protein; Ns Protein; PI-12 Protein; PI12 Protein; Serpin I1 Protein; Spi17 Protein; serpin peptidase inhibitor; clade I (neuroserpin); member 1; SerpinI1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu410

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse SERPINI1 (O35684)(Met 1-Leu 410) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SerpinI1 recombinant protein

Background: Neuroserpin, also known as Protease inhibitor 12 and SERPINI1, is a secreted protein that belongs to the serpin family. Neuroserpin is a serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. Serine protease inhibitors of the serpin superfamily are involved in many cellular processes. Neuroserpin was first identified as a protein secreted from the axons of dorsal root ganglion neurons. Neuroserpin is predominantly expressed in the brain, and is expressed in the late stages of neurogenesis during the process of synapse formation. Overexpression of neuroserpin in an anterior pituitary corticotroph cell line results in the extension of neurite-like processes, suggesting that neuroserpin may play a role in cell communication, cell adhesion, and/or cell migration. Neuroserpin may be involved in the formation or reorganization of synaptic connections, as well as synaptic plasticity in the adult nervous system. Neuroserpin may also protect neurons from cell damage by tissue-type plasminogen activator. Defects of neuroserpin are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B).

References

Schrimpf SP. et al., 1997, Genomics. 40 (1): 55-62. Hill RM. et al., 2002, Ann N Y Acad Sci. 971: 406-15. Yepes M. et al., 2004, Thromb. Haemost. 91 (3): 457-64. Galliciotti G. et al., 2006, Front Biosci. 11: 33-45.

NCBI and Uniprot Product Information

• NCBI GI #: 6678091
• NCBI GeneID: 20713
• NCBI Accession #: NP_033276.1
• NCBI GenBank Nucleotide #: NM_009250.2
• UniProt Accession #: O35684; Q543F7; Q922U6
• Molecular Weight: 160,000
• NCBI Official Full Name: neuroserpin
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade I, member 1
• NCBI Official Symbol: Serpini1
• NCBI Official Synonym Symbols: Ns; PI12; PI-12; Spi17; AI837402
• NCBI Protein Information: neuroserpin; serpin I1; protease inhibitor 17; peptidase inhibitor 12; serine protease inhibitor 17
• UniProt Protein Name: Neuroserpin
• Protein Family: Neuroserpin
• UniProt Gene Name: Serpini1
• UniProt Synonym Gene Names: Pi12; Spi17; PI-12
• UniProt Entry Name: NEUS_MOUSE

Uniprot Description

SERPINI1: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B). FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity

Biological Process: regulation of cell adhesion; negative regulation of peptidase activity