Typical Testing Data/Standard Curve (for reference only)
GuineaPig Pulmonary surfatcant-associated protein A, SP-A ELISA Kit | SP-A elisa kit
Guinea Pig Pulmonary surfatcant-associated protein A, SP-A ELISA Kit
Gene Names
Sftpa1; SP-A; Sftp1; Sftp-1
Reactivity
GuineaPig
Synonyms
Pulmonary surfatcant-associated protein A; SP-A; Guinea Pig Pulmonary surfatcant-associated protein A; SP-A ELISA Kit; Guinea Pig Pulmonary surfatcant-associated protein A (SP-A) ELISA Kit; SP-A ELISA Kit (Guinea; SP-A elisa kit
Reactivity
GuineaPig
Assay Type
Sandwich
Preparation and Storage
Store all reagents at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SP-A elisa kit
Intended Uses: This SP-A ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SP-A in the sample, this SP-A ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SP-A concentration. The concentration of SP-A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This SP-A enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for SP-A. Standards or samples are then added to the microtiter plate wells and SP-A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SP-A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SP-A are added to each well to "sandwich" the SP-A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain SP-A and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Principle of the Assay: This SP-A enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for SP-A. Standards or samples are then added to the microtiter plate wells and SP-A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SP-A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SP-A are added to each well to "sandwich" the SP-A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain SP-A and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Product Categories/Family for SP-A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,157 Da
NCBI Official Full Name
SP-A
NCBI Official Synonym Full Names
surfactant associated protein A1
NCBI Official Symbol
Sftpa1
NCBI Official Synonym Symbols
SP-A; Sftp1; Sftp-1
NCBI Protein Information
pulmonary surfactant-associated protein A; PSAP; PSP-A; surfactant pulmonary associated protein A1
UniProt Protein Name
Pulmonary surfactant-associated protein A
UniProt Gene Name
Sftpa1
UniProt Synonym Gene Names
Sftp-1; Sftp1; Sftpa
UniProt Entry Name
SFTPA_MOUSE
Uniprot Description
SFTPA2: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Defects in SFTPA2 are a cause of pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. Belongs to the SFTPA family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: multivesicular body; extracellular space; rough endoplasmic reticulum; cytoplasmic vesicle
Biological Process: positive regulation of phagocytosis
Research Articles on SP-A
Similar Products
Product Notes
The GuineaPig SP-A sftpa1 (Catalog #AAA730275) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA730275 ELISA Kit recognizes GuineaPig SP-A. It is sometimes possible for the material contained within the vial of "Pulmonary surfatcant-associated protein A, SP-A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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