Rabbit PTK6 ELISA Kit | PTK6 elisa kit

Rabbit PTK6 (Protein Tyrosine Kinase 6) ELISA Kit

Cat. Num. AAA2516113

• Gene Names: PTK6; BRK
• Reactivity: Rabbit
• Synonyms: PTK6; Rabbit PTK6 (Protein Tyrosine Kinase 6) ELISA Kit; PTK6 elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinantRabbitPTK6. No significant cross-reactivity or interference between RabbitPTK6 and analogues was observed.
• Sequence Length: 451

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTK6 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RabbitPTK6concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PTK6. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PTK6and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PTK6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PTK6.You can calculate the concentration of PTK6in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5174647
• NCBI GeneID: 5753
• NCBI Accession #: NP_005966.1
• NCBI GenBank Nucleotide #: NM_005975.3
• Molecular Weight: 14,465 Da
• NCBI Official Full Name: protein-tyrosine kinase 6 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine kinase 6
• NCBI Official Symbol: PTK6
• NCBI Official Synonym Symbols: BRK
• NCBI Protein Information: protein-tyrosine kinase 6; PTK6 protein tyrosine kinase 6; breast tumor kinase; protein-tyrosine kinase BRK; tyrosine-protein kinase BRK
• UniProt Protein Name: Protein-tyrosine kinase 6
• Protein Family: Protein-tyrosine kinase
• UniProt Gene Name: PTK6
• UniProt Synonym Gene Names: BRK
• UniProt Entry Name: PTK6_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Brk: a tyrosine kinase of the Src family. May function as an intracellular signal transducer in epithelial tissues. Very high level expression in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Selectively expressed in breast tumors and cell lines, and perhaps in colon and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Overexpression in mammary cells leads to EGF sensitization and results in a partially transformed phenotype. Enhances anchorage-independent growth and responsiveness to EGF. RNAi reduces proliferation in breast cancer cells. Kinase-inactive mutant indicates tumor function may be independent of catalytic function.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; Protein kinase, TK; TK group; Src family

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: nucleoplasm; ruffle; extrinsic to internal side of plasma membrane; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell migration; protein amino acid autophosphorylation; innate immune response; tyrosine phosphorylation of Stat3 protein; actin cytoskeleton organization and biogenesis; tyrosine phosphorylation of Stat5 protein; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; negative regulation of growth