Rabbit PTK6 Polyclonal Antibody | anti-PTK6 antibody

PTK6 Polyclonal Antibody

Cat. Num. AAA2527682

• Gene Names: PTK6; BRK
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: PTK6; Polyclonal Antibody; PTK6 Polyclonal Antibody; BRK; anti-PTK6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1.4mg/mL (varies by lot)
• Sequence Length: 451

• Applicable Applications for anti-PTK6 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:25-1:100
• Immunogen: Recombinant protein of human PTK6
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PTK6 Polyclonal Antibody at dilution 1:30)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PTK6 Polyclonal Antibody at dilution 1:30)

Related Product Information for anti-PTK6 antibody

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 5174647
• NCBI GeneID: 5753
• NCBI Accession #: NP_005966.1
• NCBI GenBank Nucleotide #: NM_005975.3
• Molecular Weight: 14,465 Da
• NCBI Official Full Name: protein-tyrosine kinase 6 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine kinase 6
• NCBI Official Symbol: PTK6
• NCBI Official Synonym Symbols: BRK
• NCBI Protein Information: protein-tyrosine kinase 6; PTK6 protein tyrosine kinase 6; breast tumor kinase; protein-tyrosine kinase BRK; tyrosine-protein kinase BRK
• UniProt Protein Name: Protein-tyrosine kinase 6
• Protein Family: Protein-tyrosine kinase
• UniProt Gene Name: PTK6
• UniProt Synonym Gene Names: BRK
• UniProt Entry Name: PTK6_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Brk: a tyrosine kinase of the Src family. May function as an intracellular signal transducer in epithelial tissues. Very high level expression in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Selectively expressed in breast tumors and cell lines, and perhaps in colon and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Overexpression in mammary cells leads to EGF sensitization and results in a partially transformed phenotype. Enhances anchorage-independent growth and responsiveness to EGF. RNAi reduces proliferation in breast cancer cells. Kinase-inactive mutant indicates tumor function may be independent of catalytic function.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: nucleoplasm; ruffle; extrinsic to internal side of plasma membrane; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell migration; protein amino acid autophosphorylation; tyrosine phosphorylation of Stat3 protein; innate immune response; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation; tyrosine phosphorylation of Stat5 protein; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of growth; regulation of cell proliferation