Rabbit anti-Human WRN Polyclonal Antibody | anti-WRN antibody

Anti-WRN Antibody (aa787-816)

Cat. Num. AAA2401824

• Gene Names: WRN; RECQ3; RECQL2; RECQL3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Protein A purified
• Synonyms: WRN; Polyclonal Antibody; Anti-WRN Antibody (aa787-816); Mouse Monoclonal (IgG1) to Human WRN; Exonuclease WRN; RecQ protein-like 2; RECQL2; RECQL3; Werner syndrome; RECQ3; DNA helicase; RecQ-like type 3; anti-WRN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This WRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 787-816 amino acids from the Central region of human WRN.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 1432

• Applicable Applications for anti-WRN antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; IHC-P: 1:100; WB: 1:1000
• Conjugation: Unconjugated
• Epitope: aa787-816
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(WRN Antibody (Center T802) western blot of K562 cell line lysates (35 ug/lane). The WRN antibody detected the WRN protein (arrow).)

Flow Cytometry (FC/FACS)

(WRN Antibody (Center T802) flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-WRN antibody

enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a5'-overhang. Has no nuclease activity towards single-stranded DNA orblunt-ended double-stranded DNA. Binds preferentially to DNA substratescontaining alternate secondary structures, such as replication forks and Holliday junctions.

NCBI and Uniprot Product Information

• NCBI GI #: 110735439
• NCBI GeneID: 7486
• NCBI Accession #: NP_000544.2
• NCBI GenBank Nucleotide #: NM_000553.5
• UniProt Accession #: Q14191; A1KYY9
• Molecular Weight: 162,461 Da
• NCBI Official Full Name: Werner syndrome ATP-dependent helicase
• NCBI Official Synonym Full Names: Werner syndrome RecQ like helicase
• NCBI Official Symbol: WRN
• NCBI Official Synonym Symbols: RECQ3; RECQL2; RECQL3
• NCBI Protein Information: Werner syndrome ATP-dependent helicase
• UniProt Protein Name: Werner syndrome ATP-dependent helicase
• Protein Family: Werner syndrome ATP-dependent helicase
• UniProt Gene Name: WRN
• UniProt Synonym Gene Names: RECQ3; RECQL2; RecQ3

NCBI Description

This gene encodes a member of the RecQ subfamily of DNA helicase proteins. The encoded nuclear protein is important in the maintenance of genome stability and plays a role in DNA repair, replication, transcription and telomere maintenance. This protein contains a N-terminal 3' to 5' exonuclease domain, an ATP-dependent helicase domain and RQC (RecQ helicase conserved region) domain in its central region, and a C-terminal HRDC (helicase RNase D C-terminal) domain and nuclear localization signal. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by accelerated aging and an elevated risk for certain cancers. [provided by RefSeq, Aug 2017]

Uniprot Description

Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (). Plays a role in double-strand break repair after gamma-irradiation.