Rabbit STAT4 Polyclonal Antibody | anti-STAT4 antibody

STAT4 (phospho-Tyr693)

Cat. Num. AAA191059

• Gene Names: STAT4; SLEB11
• Applications: Immunoblot, Western Blot, Immunohistochemistry
• Synonyms: STAT4; Polyclonal Antibody; STAT4 (phospho-Tyr693); anti-STAT4 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human, mouse, and rat STAT4 only when phosphorylated at tyrosine 693.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 748

• Applicable Applications for anti-STAT4 antibody: Immunoblotting (IB), Immunohistochemistry (IHC)
• Application Notes: Immunoblotting: use at dilution of 1:500-1:1,000. A band of~85kDa is detected.; Immunohistochemistry: use at dilution of 1:50- 1:100.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation site of tyrosine 693 (K-G-Y(p)-V-P) derived from human STAT4 and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term use.

Immunohistochemistry (IHC)

Testing Data

Related Product Information for anti-STAT4 antibody

Signal transducers and activators of transcription (STATs) are transcription factors that play important roles in signaling pathways induced by many different cytokines and growth factors that regulate cell growth and differentiation, the immune response, antiviral activity, and homeostasis. Their activation and mechanisms of action are examples of direct signaling to the nucleus by extracellular polypeptide ligands without the involvement of second messengers, tyrosine phosphorylation of transcription factors, or transcription factors that make direct contact with membrane-bound receptors and DNA. Since the discovery of STAT 1 and 2, the family has been expanded to seven members.

NCBI and Uniprot Product Information

• NCBI GI #: 4507255
• NCBI GeneID: 6775
• NCBI Accession #: NP_003142.1
• NCBI GenBank Nucleotide #: NM_003151.3
• UniProt Accession #: Q14765; Q96NZ6
• Molecular Weight: 85,941 Da
• NCBI Official Full Name: signal transducer and activator of transcription 4
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 4
• NCBI Official Symbol: STAT4
• NCBI Official Synonym Symbols: SLEB11
• NCBI Protein Information: signal transducer and activator of transcription 4
• UniProt Protein Name: Signal transducer and activator of transcription 4
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT4

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

STAT4: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q32.2-q32.3

Cellular Component: nucleoplasm

Molecular Function: protein binding

Biological Process: JAK-STAT cascade

Disease: Systemic Lupus Erythematosus, Susceptibility To, 11