Gankyrin Recombinant Protein | PSMD10 recombinant protein

Gankyrin, 1-226aa, Human,E Coli

Cat. Num. AAA203243

• Gene Names: PSMD10; p28; p28(GANK); dJ889N15.2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Gankyrin; 1-226aa; Human; E Coli; proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1; ankyrin repeat protein p28; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; PSMD10.; PSMD10 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in phosphate buffered saline (pH 7.4), 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG
• Sequence Length: 226

• Applicable Applications for PSMD10 recombinant protein: SDS-PAGE
• Preparation and Storage: Can be stored at 4°C short term (1-2 weeks).; For long term storage, aliquot and store at -20°C or -70° C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PSMD10 recombinant protein

Gankyrin is an oncoprotein commonly overexpressed in most hepatocellular carcinomas. Gankyrin interacts with S6 ATPase of the 19S regulatory particle of the 26S proteasome. It is known to play a role in the regulation of the phosphorylation of the retinoblastoma protein by CDK4, and to enhance the ubiquitinylation of p53 by MDM2. Gankyrin consists of 7 ankyrin repeats and is structurally similar to I kappa Bs. Recombinant human Gankyrin was expressed in E Coli and purified by conventional chromatography techniques.

Product Categories/Family for PSMD10 recombinant protein

Cancer

References

umemura A., et al. (2008) Hepatology. 47 (2):493-502.; Chen Y., et al. (2007) Cell Res. 17 (12): 1020-9;

NCBI and Uniprot Product Information

• NCBI GI #: 4506217
• NCBI GeneID: 5716
• NCBI Accession #: NP_002805
• NCBI GenBank Nucleotide #: NM_002814.3
• UniProt Accession #: O75832; Q5U0B2; Q8IZK9
• Molecular Weight: 24.4kDa (226aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 10 isoform 1
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 10
• NCBI Official Symbol: PSMD10
• NCBI Official Synonym Symbols: p28; p28(GANK); dJ889N15.2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 10
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 10
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD10

NCBI Description

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]

Uniprot Description

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.